Magnetic particles coupled

Solid phase Using antibodies coupled to plastic tubes, beads, micro-plates Using antibodies coupled to magnetic particles... 

We consider a set of N localized spin-j particles coupled through exchange interaction J and subject to an external magnetic field of strength B. In this section we... 

At present, various coated magnetic particles are offered on market [29-32]. Proteins have been coupled covalently to the surface of... 

In the traditional format, screen-printed electrodes are used as solid phase to carry out the immunoassay and as signal transducers. In recent years, different kinds of beads (glass, graphite particles or magnetic particles) have been used as solid phase to perform the immunoassay. For this reason, it is possible to use a new approach in the immunosensor construction coupling beads to screen-printed electrodes. 

For binding protein and human serum albumin to carboxylic acid-terminated magnetic particles was used carbodiimide coupling method. 

One of the first applications of magnetic separation in bioanalysis involves immunoassays (for a review, see Pour Carzaneh et al. (111). Here one specie of the immune couple is immobilized onto the magnetic particle and magnetic separation is performed after formation of an antigen-antibody complex. After proper washing, dissociation of the complex was performed before reading the outcome from the analysis as the amount of bound and then liberated material. 

A valid alternative to this method is now under development, and makes use of antibodies coupled to magnetic particles, so that cells expressing a specific antigen can be sorted by a magnet. 

Van Leemputten E and Horisberger M. Immobilization of enzymes on magnetic particles. Biotechnol. Bioeng. 1974 16 385-396. lost R, Miron T, and Wilchek M. The mode of adsorption of proteins to aliphatic and aromatic amines coupled to cyanogen bromide-activated agarose. Biochim. Biophys. Acta 1974 362 75-82. 

Yoshino T, Takahashi M, Takeyama H et al (2004) Assembly of G protein-coupled receptors onto nanosized bacterial magnetic particles using Mmsl6 as an anchor molecule. Appl Environ Microbiol 70 2880-2885... 

A different approach based on the use of an enzyme as a traditional amplifying label and aptamer-functionalized magnetic beads has been developed and applied to the detection of thrombin directly into human plasma (Centi et al., 2007b). The assay is based on electrochemical transduction coupled to magnetic particles,... 

Figure 8.4 Analysis of thrombin-spiked serum (A) and plasma (B) samples by aptamer-based electrochemical sandwich assay coupled to magnetic particles. [From Centi...

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