Macromolecular content

COMPND (describes the macromolecular contents of the entry, and EXPDTA (identifies the technique used to determine the JD structure of the compound experimentally). 

The fir.-fit line of the file (see Figure 2-110) - the HEADER record - hold.s the moleculc. s classification string (columns 11-50), the deposition date (the date when the data were received by the PDB) in columns 51-59, and the PDB (Dcode for the molecule, which is unique within the Protein Data Bank, in columns 63-66. The second line - the TITLE record - contains the title of the experiment or the analysis that is represented in the entry. The subsequent records contain a more detailed description of the macromolecular content of the entiy (COMPND), the biological and/or chemical source ofeach biological molecule in the entiy (SOURCE), a set ofkeywords relevant to the entiy (KEYWDS). information about the experiment (EXPDTA), a list of people responsible for the contents of this entiy (.AUTHOR), a history of modifications made to this entiy since its release (REVDAT), and finally the primaiy literature citation that describes the experiment which resulted in the deposited dataset ()RNL). 

Macromolecular synthesis The macromolecular contents increased in all of the mutants. The Total DNA content (Table II) was observed to be higher in the edifenphos resistant mutants. UV-induced . oryzae mutants showed only 20% increase of DNA over sensitive strain. Similarly D oryzae mutants also showed an increase in the DNA content. Among the phospholipids the increase in phosphatidylcholine was significant in the resistant mutants. 

Table II. Macromolecular content of sensitive strain and resistant mutants of P. oryzae and IK oryzae...

Various cellular contents analysed,showed significant increase in all the macromolecular contents viz. DNA, RNA Protein, lipids and phospholipids of the EDDP-resistant mutants of P. oryzae and IK oryzae. The efflux of electrolytes was reduced in the resistant mutants when compared to control. In interpreting the results, it should be realized that the phospholipids, the target site of inhibition for edifenphos seem to be unaffected in the mutants as evidenced from the higher values of phosphatidylcholine compared sensitive strain. These data indicate that the mechanism of resistance to edifenphos is not related to the target site of edifenphos. De waard and Van Nistelrooy(38) also reported that the mechanism of resistance in J . oryzae to pyrazophos (PP) was not related to the change of target site of PP. It seems probable that resistance in P oryzae and IK oryzae to edifenphos... 

Turner K, Porter J, Pickup R, Edwards C (2000) Changes in viability and macromolecular content of long-term batch cultures of Salmonella typhimurium measmed by flow cytometry. 

Dry weight, protein, carbohydrate and RNA increased in a linear manner throughout the light period. DNA remained constant until two hours before the dark period when it doubled. As cell division occurred, the macromolecular content of these parameters returned to the initial values obtained at the beginning of the light period (TABLE 1). No significant differences at the 0.05% level were observed between the media or 0.05% acetone controls. 

FIGURE 1 Effect of 5 mg L of MataciH.SD (1/2.5/1.5 mg L of Aminocarb, nonylphenol, and diluent oil, respectively) and nonylphenol alone on the dry weight and macromolecular content of Chlamydomonas cells when applied at 2 hr before the dark period. The mean values of triplicate samples are shown together with standard deviation bars. 

Irrechukwu, O. N., P.-C. Lin. et al. 2010. Magnetic resonance studies of macromolecular content in engineered cartilage treated with pulsed low-intensity ultrasound. Tissue Eng Part A 17(3-4) 407-415. 

An alternative and much more flexible approach is represented hy the STAR file format [L48, 149, which can be used for building self-describing data files. Additionally, special dictionaries can be constructed, which specify more precisely the contents of the eorresponding data files. The two most widely used such dictionaries (and file formats) arc the CIF (Crystallographic Information File) file format [150] - the International Union of Crystallography s standard for representation of small molecules - and mmCIF [151], which is intended as a replacement for the PDB format for the representation of macromolecular structures,... 

Alzheimer s Disease. Figure 1 A(3 monomers can self-associate to form dimers, trimers and higher oligomers. Globular structures of synthetic A(342 are known as A(3-derived diffusible ligands (ADDLs) (3-12-mers of A(3). These structures are similar to the smallest protofibrils and represent the earliest macromolecular assembly of synthetic A(3. The characteristic amyloid fiber exhibits a high beta-sheet content and is derived in vitro by a nucleation-dependent self-association and an associated conformational transition from random to beta-sheet conformation of the A(3 molecule. Intermediate protofibrils in turn self-associate to form mature fibers. 

During the in vitro fermention the amount of macromolecular pectin was diminished continuously. On the other hand the fraction of OligoGalA was increased at first and diminished later. The content of short chain fatty acids, which are typical end products of fermentation of dietary fibers rised permanently (Figure 5). Low-esterified pectins were fermented in vitro faster by human faecal flora than the high-esterified pectins. 

Pharmacology Sodium ferric gluconate complex in sucrose injection is a stable macromolecular complex used to replete the total body content of iron. 

With the continuously increasing usage of X-ray crystallography, the number of macromolecular structures deposited in the PDB, Fig. 11.4, their size, and complexity are rapidly growing. Particularly stuiming is the increase in the volume (and the content) of the crystallographic asymmetric unit. 

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