Lysozyme Malat

The previously discussed enzymes may all be used for solid-phase AM assays. For homogeneous AM-type EIA, lysozyme, malate dehy-rogenase (MDase), glucose-6-phosphate dehydrogenase (GPDase), and ribonuclease A (RNase A), are used in addition to BGase. 

RNS, ribonuclease LZM, lysozyme SNS, staphylococcal nuclease LZ4, T4 lysozyme PAP, papain TLS, thermolysin, TRX, thioiedoxin FLN, flavodoxin ADH, alcohol dehydrogenase coenzyme domain AKN, adenyl kinase MDG, malate dehydrogenase TIM, triosephosphate isomerase SUB, subtilisin CPA, carboxypeptidase LDH, lactate dehydrogenase PGK, phosphoglycerate kinase GPD, glyceraldehyde 3-phosphate dehydrogenase, HKN, hexokinase. 

Various enzymes, lysozyme, catalase, phosphatase, malate/formate dehydrogenase, adenylase kinase, pymvate decarboxylase, alkaline phospatase, serum albumin, lipoprotein, interferon, growth factor, polypeptide hormone DNA, RNA, y-globulin, proteinase, carboxypeptidase, endotoxins, pyrogen, IgG, trypsin inhibitor, transferrin, casein, L-benzoyl arginine ethyl ester, cytocrome C IgG, heparin... 

Lactate dehydrogenase Malate dehydrogenase Alkaline phosphatase Ribonuclease A Lysozyme Glucose oxidase Ribonuclease B Peroxidase (his)n Glucose oxidase (his)n /1-galactosidase (his)6... 

In a schematic elution pattern of some standard proteins, peroxidase was eluted first with saline, BSA came next with glycine buffer at pH 6.6 and hemoglobin and catalase were eluted at a pH of nearly 8.0. Aldolase, lysozyme, chymotrypsinogen A, malate dehydrogenase, and cytochrome c were not eluted under these conditions, but were eluted with 0.1% SDS. The adsorption order does not depend on the isoelectric point, the molecular mass, or the content of basic amino acids. However, adsorption may depend on the o -helix content, and the secondary structure of those proteins may be important. We have also reported on protein adsorption and separation on siliconized glass surfaces (30), and on the adsorption and separation of nucleic acids on those same surfaces (31-35). 

Various enzymes, lysozyme, catalase, phosphatase, malate/formate dehydrogenase, adenylate kinase, pyruvate decarboxylase, alkaline phosphatase, serum albumin, lipoprotein, interferon, growth factor, polypeptide hormone... 

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