Lysozyme in water

Enzymes are sometimes more stable in anhydrous organic solvents than in water. Lysozyme in water at 100 °C was demonstrated to be 50% deactivated after 30 s (pH 8) or after 100 min (pH 4), but after 140 h in cyclohexane or even after 200 h as a dry powder (Zaks, 1986a). 

By direct absorption measurements, we have directly probed the low frequency collective motions of proteins in water by terahertz absorption spectroscopy. We have isolated, for the first time, the 0.075 - 3.72 THz absorption of bovine semm albumin " and hen egg white lysozyme in water, and made direct comparison with existing... 

J. Xu, K. W. Plaxco and S. J. Allen, Collective dynamics of lysozyme in water terahertz absorption spectroscopy and comparison with theory, submitted. 

The values of the force field parameters need to be adjusted to ensure that the coarse-grained model gives reasonable results in comparison with experiment. Tables 4, 5, and 6 show the values of C, and e obtained from the PMFs for the 210 bead-bead pairs. Here, we select the osmotic second virial coefficient (B22) of lysozyme as the reference property because it well represents the strength of lysozyme-lysozyme interactions in a solution. Lenhoff and his colleagues [41 14] measured B22 for lysozyme in a variety of solutions. Lysozyme is expected to have a positive B22 in water because it is positively charged. Their data indicate that B22 of lysozyme in water at pH 7 is around 5 x 10 mol rnl/g. ... 

Fig. 12.26 The vibrational Raman spectrum of lysozyme in water. (From Raman spectroscopy,...

Thus, investigation of the kinetics of lysozyme-catalyzed lysis of bacterial cell walls has shown that the presence of an organic solvent decreased the overall reaction rate reversibly with the rados of the maximum rate constant of lysis in water and in mixed solvent given by... 

Obviously, the spectra are more sharply defined than at 20°C. They are quite stable over several hours. Difference spectra have been recorded in 40% methanol between pH 2.0 and 10.6 at -20°C and are shown in Fig. 5. There is a real peak at 298 nm between pH 4.7 and 6.1, which could correspond to carboxyl group ionization. The amplitude AOD = ODj94 - OD291 is pH dependent and the profile of substrate binding in 40% methanol at -20°C shows maximum binding at about pH 4.7, which compares well to results obtained in water by Banerjee and Rupley (1973). Thus, difference spectra obtained on mixing lysozyme and hexa-NAG in mixed solvents at room as well as at subzero tempera-... 

Sodium dodecyl sulfate (SDS), 25% in water Lysozyme solution, 10 mg/mL in water Sodium perchlorate, 5 M Chloroform-isoamyl alcohol, 24 1 95% ethanol... 

Figure 25-29 Carbon-13 nmr spectrum at 45.3 MHz of lysozyme, 0.015M in water solution, taken with proton decoupling (Section 9-10L)...

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