Lysine sensor

The lysine sensor reported by Karube et al. was a micro-C02 sensor containing immobilized L-lysine decarboxylase (LDC, Fig. 3.18.C). L-lysine catalysed the following reaction... 

Biosensors are also available for glucose, lactate, alcohol, sucrose, galactose, uric acid, alpha amylase, choline, and L-lysine. All are amperometric sensors based on O2 consumption or H2O2 production in conjunction with the turnover of an enzyme in the presence of substrate. In the case of glucose oxidase reaction, the normal biological reaction is ... 

N. Garcia-Villar, J. Saurina and S. Hernandez-Cassou, Potentiometric sensor array for the determination of lysine in feed samples using multivariate calibration methods, Fresenius J. Anal. Chem., 371(7) (2001) 1001-1008. 

Lysine aminoacid+interferents 7 PVC sensors+biosensor — Animal feeds [74]... 

Fig. 4.12 Dansyl-decorated oligo-lysine dendrimer (2nd generation) as dendritic antenna with sensor properties towards metal ions (according to Balzani, Vogtle et al)...

Luz, R. D. S., Damos, F. S., Tanaka, A. A. and Kubota, L. T. (2006), Dissolved oxygen sensor based on cobalt tetrasulphonated phthalocyanine immobilized in poly-L-lysine film onto glassy carbon electrode. Sensor Actuator B Chem., 114(2) 1019-1027. 

Lysine Glucose Production process monitoring using a 13C-sensor reactor coupled to the production process [106]... 

Lysine Glucose Fructose Serial flux mapping of fed-batch cultures using the sensor reactor approach [109]... 

Enzymes have been attached to a nylon matrix (135-13 7), a pig intestine (138), the hydrophobic membrane of a gas-selective sensor (139), and controlled pore glass (140). Recent comparative studies of the coupling agents GA and benzoquinone support the preferential use of benzoquinone for binding GOD to nylon mesh (13 7), or to cellulose acetate membranes (141) with lysine (13 7). Both investigations report robust electrode behaviors with respect to prolonged exposure to glucose, while lifetimes of the membrane electrodes were ca. 3 months. 

Chemically binding enzymes to nylon net is very simple and gives strong mechanically resistant membranes (135). The nylon net is first activated by methylation and then quickly treated with lysine. Finally, the enzyme is chemically bound with GA. The immobilized disks are fixed direcdy to the sensor surface or stored in a phosphate buffer. GOD, ascorbate oxidase, cholesterol oxidase, galactose oxidase, urease, alcohol oxidase (135), and lactate oxidase (142) have been immobilized by this procedure and the respective enzyme electrode performance has been established. 

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