Lymphocyte around tumor cells

As shown in Fig. (5) , primary tumor cells in the spleen grew and invaded the while and red pulp of the spleen in mice with intrasplenically implanted LLC. The orally administered triterpenoid fractions (100 or 200 mg/kg) prevented the tumor growth and the invasion of the white pulp of the spleen. Furthermore, the orally administered triterpenoid fractions induced an accumulaiion of lymphocytes around tumor cells in the white pulp of the spleen Fig. (5) . The metastatic tumor cells in the liver also grew and invaded the hepatocytes in mice with intrasplenically implanted... 

FIGURE 1.19 (A, B) B5-fixed section of bone marrow aspirate depicting a small solitary nodule consisting of lymphocytes and occasional plas-macytoid lymphocytes, with scattered plasma cells around it. Many of the lymphoid and plasmacytoid cells reacted strongly with anti-lambda antibody (A). Anti-kappa reacted only with scattered plasma cells outside the nodule (B, small black dots). This patient eventually was shown to have a solitary plasmacytoid lymphocytic lymphoma in the small intestine (also lambda type). Paraffin sections, DAB with hematoxylin counterstain (xl 25). There was no serum paraprotein detectable at this time presumably the number of tumor cells was insufficient for production of a detectable paraprotein however, a "monoclonal" IgM appeared later in the course. From Taylor CR, Cote Rj. Immunomicroscopy A Diagnostic Tool for the Surgical Pathologist 2nd ed. Philadelphia WB Saunders 1994 59. 

Participation of the T-cell subsets was also examined histochemically in subcutaneously rechallenge tumor sections, Fig. (6)-Protocol C. The accumulation of lymphocytes in the tumor tissues and around the tumor increased in the CVS-treated group, 2 days after the s.c. tumor rechallenging, Fig. (8)-B. Immunohistochemical staining revealed increased infiltration of mature CD3 (D), CD4 (F), and CD8 (H) -positive T-cell s into the tissue in the CVS group. The tumor mass disappeared in about 50 percent of the CVS-treated mice 7 days after tumor inoculation, while in all control PBS mice it increased progressively until death (data not shown). 

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