Lymphoblastoid cells analysis

Joubert-Caron R et al. Protein analysis by mass spectrometry and sequence database searching a proteomic approach to identify human lymphoblastoid cell line proteins. Electrophoresis 2000 21 2566— 2575. 

Toda T et al. Proteomic analysis of Ep-stein-Barr virus-transformed human B-lymphoblastoid cell lines before and after immortalization. Electrophoresis 2000 21 1814-1822. 

Steen, A.-M., Meyer, K.G Recio, L. (1997a) Analysis of hprt mutations occurring in human TK6 lymphoblastoid cells following exposure to 1,2,3,4-diepoxybutane. Mutagenesis, 12, 61-67... 

Joubert-Caron, R., Le Caer, J.P., Montandon, F., Poirier, F., Pontet, M., Imam, N., Feuillard, J., Bladier, D., Rossier, J., and Caron, M., 2000, Protein analysis by mass spectrometry and sequence database searching a proteomic approach to identify human lymphoblastoid cell line proteins [In Process Citation]. Electrophoresis 21 2566-2575. 

Lester, E.P., Lemkin, P., and Lipkin, L., 1982, A two-dimensional gel analysis of autologous T and B lymphoblastoid cell lines. Clin. Chem. 28 828-839. 

Tissot, J.D., Vu, D.H., Schneider, P., Vuadens, F., Crettaz, D., and Duchosal, M.A., 2002b, The immunoglobulinopathies From physiopathology to diagnosis. Proteomics 2 813-824 Toda, T., Sugimoto, M., Omori, A., Matsuzaki, T., Furuichi, Y., and Kimura, N., 2000, Proteomic analysis of Epstein-Barr virus-transformed human B- lymphoblastoid cell lines before and after immortalization. Electrophoresis 21 1814-1822. 

In later TEM studies by Ghadially et al. (48), a lower concentration of cisplatin (30 (xM) was incubated in HeLa and human lymphoblastoid (RPMI 6410) cells for periods that ranged from 1 hour to 4 days. No intracellular platinum was detected in either cell line. However, similar incubations with platinum(II)-uracil resulted in development of lysosome-like bodies in the cytosol, which are referred to as platinosomes, that contain electron dense species identified by X-ray analysis as platinum (48). In a related study, similar concentrations of cisplatin were injected into rabbit knee joints and incubated for several days. Again, no platinum was detected in the intracellular or extracellular compartments of the synovial cells, whereas platinum was observed to accumulate only in the platinosomes after platinum-uracil incubation (49). This set of studies highlights the effect of incubation concentrations of the drug on the cellular distribution results, as well as, drawing attention to the uptake of some platinum(II) complexes in cytoplasmic organelles. 

Stacey, G. N Bolton, B. J., Morgan, D., Clark, S. A., and Doyle, A. (1992) Multilocus DNA fingerprint analysis of cell banks Stability and culture identification in human B lymphoblastoid and mammalian cell stocks. Cytotechnology 8,13-20. 

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