Luminometric methods

Hermann (2000) described a rapid automated method involving generation of a known amount of free radicals and the detection of the excess by photochemiluminescence. Test kits are available for determination of total water-soluble antioxidants, fat-soluble antioxidants and ascorbic acid. A luminometric method was developed for the determination of antioxidative activity and was subsequently applied to anthocyanin and betalaine colour concentrates (Kuchta et al., 1999). The method involved quantification of the interruption in luminescence from the hydrogen peroxide-horse radish peroxidase-luminol system in the presence of antioxidants. 

R. Giangiacomo, R. Lizzano, S. Barzaghi, T. M. R Cattaneo, A. S. Barros. NIR and other luminometric methods to monitor the primary clotting phase of miUc. J Near Infrared Spectrosc. 6 205-212, 1998. 

Decker, K.A. Hinkkanen, A. Luminometric determination of flavin adenindinucleotide. Methods Enzymol. 1986, 122, 185-192. 

To control sterility or cleanness in hospital environment microbiology methods, laborious and time consuming (24-120 h), are used at present. To accelerate and simplify the sterility or cleanness control on different surfaces we applied bioluminescent assay of total bacterial contamination (TBC). Since the most surfaces analyzed in hospital contained low number of bacteria, below the detection limit of ATP-reagent used, incubation of the samples in nutritive medium followed by filtration through special luminometric microcuvettes Filtravette was applied. 

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