Luminescence and Fluorescence Spectroscopy

Perkin Elmer and Hamilton both supply luminescence instruments (see Appendix 1). 

The Perkin Elmer LS-3B instrument is a fluorescence spectrometer with separate scarming monochromes for excitation and emission, and digital displays of both monochromator wavelengths and signal intensity. The LS-5B instrument is a rotating luminescence spectrometer with the capability of measuring fluorescence, phosphorescence, and bio-and chemiluminescence. Delay time (t ) and gate width (tj are variable via the keyboard in 10 ps intervals. The instrument collects excitation and emission spectra. 

Both instruments are equipped with a xenon discharge lamp source and have an excitation wavelength range of 230-720 nm and an emission wavelength range of 250-800 nm. 

These instruments feature keyboard entry of instrument parameters which, combined with digital displays, simplifies instrument operation. A high-output pulsed xenon lamp, having low power consumption and minimal ozone production, is incorporated within the optical module. 

Fluorescence spectrometers are equivalent in their performance to single-beam UV-visible spectrometers in that the spectra they produce are affected by solvent background and the optical characteristics of the instrument. These effects can be overcome using software built into the Perkin Elmer LS-5B instrument or using application software for use with the Perkin Elmer models 3700 and 7700 computers. 

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