Luby-Phelps

K. Luby-Phelps, P. E. Castle, D. L. Taylor, and F. Lanni, Hindered diffusion of inert tracer particles in the cytoplasm of mouse 3T3 cells, Proc. Natl. Acad. Sci. USA 84, 4910 (1987). 

Luby-Phelps K, Mujumdar S, Mujumdar RB, Ernst LA, Galbraith W, Waggoner AS (1993) A novel fluorescence ratiometric method confirms the low solvent viscosity of the cytoplasm. Biophys J 65(l) 236-242... 

Jakob CA, Burda P, Roth J, Aebi M (1998) Degradation of misfolded endoplasmic reticulum glycoproteins in Saccharomyces cerevisiae is determined by a specific oligosaccharide structure. J Cell Biol 142 1223-1233 Janson LW, Ragsdale K, Luby-Phelps K (1996) Mechanism and size cutoff for steric exclusion from actin-rich cytoplasmic domains. Biophys ) 71 1228-1234 Jeffers M, Taylor GA, Weidner KM, Omura S, Vande Woude GF (1997) Degradation of the Met tyrosine kinase receptor by the ubiquitin-proteasome pathway. Mol Cell Biol 17 799-808... 

Lin, P. Luby-Phelps, K. Stull, J.T. Properties of filament-bound myosin light chain kinase. J. Biol. Chem., 274, 5987-5994 (1999)... 

Luby-Phelps, K. (2000) Cytoarchitecture and physical properties of cytoplasm volume, viscosity, diffusion, intracellular surface area. Int. Rev. Cytol, 192. 

Provance, Jr., D.W., McDowall, A., Marko, M. and Luby-Phelps, K. (1993) Cytoarchitecture of size-excluding compartments in living cells. J. Cell Sci., 106, 565-577. 

Ragsdale GK, Phelps J, Luby-Phelps K. Viscoelastic response of fibroblasts to tension transmitted through adherens junctions. Biophys J. 1997 73 2798-2808. 

Baker, S.A., Freeman, K., Luby-Phelps, K., Pazour, G.J. and Besharse, J.C. (2003) IFT20 links kinesin II with a mammalian intraflagellar transport complex that is conserved in motile flagella and sensory cilia. J. Biol. Chem. 278, 34211-34218. 

Rozelle AL, Machesky LM, Yamamoto M, Driessens MH, In-sall RH, Roth MG, Luby-Phelps K, Marriott G, Hall A, Yin HL. Phosphatidylinositol 4,5-bisphosphate induces actin-based movement of raft-enriched vesicles through WASP-Arp2/3. Curr. Biol. 2000 10 311-320. 

Friedrich, P. (1984). Supramolecular Enzyme Organization, Pergamon Press, New York/Oxford. Luby-Phelps, K., Latuii, F., Taylor, D. L. (1988). The submicroscopic properties of cytoplasm as a determinant of cellular function. Atm. Rev. Biophys. Biophys. Chem. 17,369-396. 

Luby-Phelps, K., F. Lanni, and D. Taylor, Behavior of a fluorescent analogue of calmodulin in living 3T3 cells. Journal of Cell Biology, 1985, 101, 1245-1256. 

Hou, L., F. Lanni, and K. Luby-Phelps, Tracer diffusion in F-actin and Ficoll mixtures. Toward a model for cytoplasm. Biophysical Journal, 1990, 58, 31 3. 

Luby-Phelps and collaborators used FRAP to study the diffusion of fractionated ficolls in cellular cytoplasm and in concentrated protein solutions(108). Ficoll fractions had hydrodynamic diameters 60-250 A. The Dp/Dpo of ficolls in protein solutions was independent of the size of the ficoll molecules. That is, protein solutions are not size filters they retard to approximately the same fractional extent the diffusion of small and large probe particles. In contrast, cell cytoplasm is a size filter. The Dp/Dpo of ficolls in cytoplasm falls sixfold as the ficoll diameter is increased from 60 to 500 A. For the largest ficoll, FRAP shows that a third of the ficoll particles are trapped only 2/3 of the particles can diffuse through substantial distances. Observations that cellular interiors are size filters were also made by Arrio-Dupont et a/.(109,110). 

Hou, etal.(lll) compared their results with findings of Luby-Phelps, et a/.(108) on probes in vivo. In living cells, Dp/Dpo depends strongly on probe radius Rh... 

The evidence on probe size reduces to two general statements and a qualification. First, true gels are indeed size filters, as witness results of, e.g., Luby-Phelps,... 

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