Lobe dissection

The collected oocytes need to be individually isolated from the connective ovarian tissue and the layer of follicular cells that surround each oocyte (Goldin, 1992). For a detailed description, see Yao et al. (2000). Briefly, the excised ovarian lobe is washed in MBM and dissected using sterile forceps into smaller sections containing approximately 5-10 oocytes each. The sectioned clumps of oocytes are treated with MBM supplemented with 2 mg/mL type I collagenase (Worthington, Lakewood, NJ) for 2 h at room temperature with constant shaking to separate the oocytes from the follicular cell layer. The oocytes are subsequently washed five times with a BSA solution (bovine serum albumin, 1 mg/mL in MBM), followed immediately by five wash cycles in MBM. 

Most pulmonary tumor colonies in mice are in the pleura. Therefore, pigmented colonies (such as melanomas) can be counted without lung dissection. However, it may be more facile, especially when there are extensive pulmonary metastases, to dissect the lungs into the individual four or five lobes which are then enumerated individually using a dissecting microscope. 

Lobing and dissection (Fig. 7-llb) tend to decrease the effective length across a leaf in the direction of the wind and hence to reduce Sbl (Eq. 7.10), with a consequent increase of convective heat exchange. For instance, the heat convection coefficient hc (Eq. 7.17) increases with the depth of leaf serrations. In addition to differences in size, the greater lobing observed for sun leaves compared to shade leaves on the same plant further reduces the heating of sun leaves above air temperature. Also, heat convection is greater for a pinnate leaf with many leaflets than for a simple undivided (entire) leaf of the same area (Fig. 7-llb). 

Fig. 3. Schematic drawings depicting the procedure for dissection of pupal retinas, (a) Opening of the pupal case, (b) Removal of the pupal head from the case, (c) The retinae (fi) attached to the optic lobe (OL) and brain (fl) (For a detailed description see Pupal Retina").

Add glycerol PBS (1 1) to the tube and immediately remove the samples with a cut tip (while they are floating) and place onto a slide. Dissect out the optic lobe with needles and mount the slide with only the eye disc, or retina, on it (see Note 21). Alternatively the slides with imaginal discs can be mounted as previously described for embryos (see Staining and Mounting on Slides ). The samples can be circled with a nonfluorescent pen to facilitate viewing. 

The 30 xm rastering of the tissue (so-called oversampling technique with a < 100 xm diameter laser spot size), nicely allows for clear differential localization of peptide families, including ones exclusively localized in the ca, and others which are predominant in the cc. Within the cc, even a distinction can be made between peptide distributions over the glandular and the storage lobes. The only way this type of peptide localization data could be obtained in the past was by tedious individual dissection of the minute subparts of the little glands followed by peptide... 

Hemoptysis, hemothorax, or alveolar hemorrhage may occur suddenly. In a case of massive hemoptysis, histologic examination of a resected lower lobe revealed dissection of a medium-sized pulmonary artery containing amyloid deposits (16). 

Accessory Sex Organs Taurine was present In high concentration (Table 2). The accessory sex organs of the blue crab were the only ones which were readily dissected Into the component parts. The squid again contained an exceptionally high concentration. The concentration of taurine was distinctively different for the three components. Hypotaurlne was present at relatively low values except In the anterior lobe of the blue crab. The other sulfur amino acids also were In higher concentration In the anterior lobe of the blue crab. 

For dissecting the discs free from the body wall, tear the larva in half and invert the body wall. For dissecting the cluster of dorsal discs (wing, haltere, and second leg disc), pinch the larvae about a third of the way posterior and rip the dorsal anterior side of the larvae a little further anterior. The dorsal cluster is located about a quarter of the way posterior in a dorsolateral position, and often pops out of the resultant tear it must then be teased loose from the trachea. The other leg discs are found on the ventral side of the ventral brain. The eye-antennal discs are stretched between the surface of the optic lobes of the brain and mouth parts. The discs appear slightly more transparent than the white-yellow fat body and can be easily identified with a little practice. 

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