Liver Specific Drug Transport in Sandwich-cultured Hepatocytes

Liver Specific Drug Transport in Sandwich-cultured Hepatocytes 

The use of in vitro systems to evaluate hepatic drug uptake and efflux is an essential part of the drug development process. Primary hepatocyte culture is one technique to address this issue. 

Male Wistar rats (250-280 g) can be obtained from Charles River. They are allowed free access to food (laboratory rodent diet 5001, PMI Feeds, St. Louis, MO) and water, and are housed in a constantly alternating 12-h light (6 00 am to 6 00 pm) and dark cycle. 

3 ml DMEM to each dish and storage in a humidified incubator. 

Hepatocytes are counted in a hemocytometer and viability is determined using the trypan blue exclusion method. Viability is always 90 % with a yield of 2-3 x 10s cells. Cells are resuspended in medium and diluted to a final concteration of 1 x 106 cells/ml. 

---

II.I.4.2 Liver Specific Drug Transport in Sandwich-cultured Hepatocytes. . 540... 

---