Lipoxygenases metabolites

Hampson, AJ, Hill WA, Zan Phillips M, Makriyannis A, Leung E, Eglen, RM, Bornheim LM. Anandamide hydroxylation by brain lipoxygenase metabolite structures and potencies at the cannabinoid receptor. Biophys. Biochim. Acta 1995 1259 173-179. 

The title compounds LTB4, 21 (Z) and 22 (Z), have been synthesized23 by stereoselective reduction with deuterium gas of a 1 1 mixture of the suitable diacetylenic precursors 23 and 24 using Lindlar catalyst or palladium on barium sulphate catalyst (equation 10). Leukotriene B4, a 5-lipoxygenase metabolite of arachidonic acid, playing... 

Massey KD, Minnich BN, Burt JM Arachidonic acid and lipoxygenase metabolites uncouple neonatal rat cardiac myocyte pairs. Am J Physiol 1992 263 C494—C501. 

Figure 2 Known second-messenger pathways that modulate capsaicin or VRs. GPCR, G protein-coupled receptors PLA2, phospholipase A2 AC, adenylate cyclase IP3, inositoltriphosphate DAC diacylglycerol LM, lipoxygenase metabolites AA, arachidonic acid ANA, anandamide PG, prostaglandins Gq/11, Gq/j, Gs, trimeric G-proteins (adapted from Premkumar, 2001)...

Gardner, H.W. 1997. Analysis of plant lipoxygenase metabolites. In Advances in Lipid Methodology - Four vol. 8 (W.W. Christie, ed.) pp. 1-43. Oily Press, Dundee, Scotland. 

Pygeum africanum, an extract from the bark of the African prune tree, has been used in Europe since 1969 to treat men with mild to moderate symptoms of benign prostatic hyperplasia. The mechanism of action of P. africanum is not known. In animal models, P. africanum modulates bladder contractility, has anti-inflammatory activity, decreases production of leukotrienes and other 5-lipoxygenase metabolites, inhibits fibroblast production, affects adrenal androgens, and restores the secretory activity of prostate epithelium. 

Hampson, A. J., Hill, W. A. G., Zan-Phillips, M., Makriyannis, A., Leung, E., Eglen, R. M., and Bornheim, L. M. (1995). Anandamide hydroxylation by brain lipoxygenase Metabolite structures and potencies at the cannabinoid receptor. Biochim. Biophys. Acta 1259, 173—179. 

Liquid C h rom atography/M ass Spectrometry. Increased use of liquid chromatography/mass spectrometry (lc/ms) for structural identification and trace analysis has become apparent. Thermospray lc/ms has been used to identify by-products in phenyl isocyanate precolumn derivatization reactions (74). Five compounds resulting from the reaction of phenylisocyanate and the reaction medium were identified two from a reaction between phenyl isocyanate and methanol, two from the reaction between phenyl isocyanate and water, and one from the polymerization of phenyl isocyanate. There were also two reports of derivatization to enhance either the response or structural information from thermospray lc/ms for linoleic acid lipoxygenase metabolites (75) and for cortisol (76). 

Piomelli, D. and Greengard, P. (1990). Lipoxygenase metabolites of arachidonic acid in neural transmembrane... 

Rao GHR, Radha E, White JG. Irreversible aggregation does not depend on lipoxygenase metabolites. BiochemBiophys Res Comm 1985 132 652-657... 

Takenaga M, Hirai A, Terano T, Tamura Y, Kitagawa H and Yoshida S. (1986). Comparison < the in vitro effect of eicosapentaoioic acid (EPA) -derived lipoxygenase metabolites on human platelet function with those of arachidonic acid. Throm. Res. 37,373-384. 

Lipoxygenase metabolites of arachidonic acid such as 12-hydroxyeicosa-tetraenoic acid (12-HETE) have been shown to be involved in the release of neurotiansmitters and other cellular functions (92-94). Basal generation of 12-HETE has been reported to be about 3.7-fold greater in SHR than in WKY platelets (95,96). Arachidonic acid has been shown to stimulate platelets by activating protein kinase C, by a prostaglandin-independent mechanism (97). It is possible that the lipoxygenase metabolites of arachidonic acid may also be involved in altered reactivity in hypertension. 

Lipoxygenase metabolites of arachidonic acid as second messoigers for presfynaptk inhibition of lysia sensory cells. Nature, 328,38-43,1987. 

Platelets in hypercholesterolemic patients are more susceptible to aggregation than normal subjects [72]. Aggregation is mediated by several mediators, including cyclooxygenase and lipoxygenase metabolites of arachidonic acid [73]. A deficiency of vitamin E enhanced eicosanoid production in platelets [74,75]. 

Platelet 5-Hydroxytryptamine Lipoxygenase metabolites Platelet-activating factor Thromboxane A2... 

Haliday, E.M., Ramesha, C.S., and Ringold, G. (1991). TNP induces c-fos via a novel pathway requiring conversion of arachidonic acid to a lipoxygenase metabolite. EMBO J 10, 109-15. 

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