Lipids detergent-assisted

Once the desired mixture of lipid components is dissolved and homogenized in organic solvent, one of several techniques may be used to disperse the liposomes in aqueous solution. These methods may be broadly classified as (1) mechanical dispersion, (2) detergent-assisted solubilization, and (3) solvent-mediated dispersion. 

Bile salts Cholesterol derivatives with detergent-like properties used to solubilize cholesterol, assist in intestinal absorption of fat-soluble vitamins, and emulsify dietary lipids passing through the intestine to enable fat digestion and absorption by exposing fats to pancreatic lipases. 

Bile acid A cholesterol-derived detergent molecule, such as cholic acid, that Is secreted by the gallbladder into the intestine to assist in the absorption of dietary lipids. 

Much of our information about lipases comes from studies on the enzyme isolated from pancreatic juice this is the enzyme primarily responsible for the digestion of dietary lipids in the animal digestive tract, a process that is described in detail in section 5.3.1. Pancreatic lipase is a glycoprotein of molecular mass 50kDa. The first step in the catalytic process is the adsorption of the enzyme on to the hydrophobic interface of the substrate micelles. Detergent molecules, like bile salts, tend to compete with the lipase for binding sites and the adsorption of the enzyme is assisted by a helper molecule called co-lipase, which is a small protein. In vitro, it can be shown that the activity of lipase is inhibited by high concentrations of bile salts and that this inhibition can be overcome by the addition of co-lipase. 

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