Lipid from Salmonella typhimurium

Figure 1. A schematic diagram illustrating a basic lipopolysaccharide molecule from Salmonella typhimurium with the polysaccharide and lipid regions. Also shown are core polysaccharide mutants (Ra through Re).

Using PTLC six major fractions of lipids (phospholipids, free sterols, free fatty acids, triacylglycerols, methyl esters, and sterol esters) were separated from the skin lipids of chicken to smdy the penetration responses of Schistosoma cercaria and Austrobilharzia variglandis [79a]. To determine the structure of nontoxic lipids in lipopolysaccharides of Salmonella typhimurium, monophosphoryl lipids were separated from these lipids using PTLC. The separated fractions were used in FAB-MS to determine [3-hydroxymyristic acid, lauric acid, and 3-hydroxymyristic acids [79b]. 

On the other hand, Boons et al. demonstrated the synthesis of Rhizobium sin-1 lipid A derivatives [96, 97], E. coli hexaacylated and Salmonella typhimurium hep-taacylated lipid A [98], tetraacylated derivatives of lipid A from Porphyromonas gingivalis [99], and lipid A derivatives and KDO-linked lipid A of Neisseria meningitidis [100],... 

Qureshi, N., Takayama, K., Ribi, E. Purification and structural determination of nontoxic lipid A obtained from the lipopolysaccharide of Salmonella typhimurium. J Biol Chem 257 (1982) 11808-11815. 

Qureshi, N., Takayama, K., Ribi, E. Purification and structural determination of non-toxic lipid-A from lipopolysaccharide of Salmonella typhimurium. J Biol Chem 257 (1982) 11808-11815. Raetz, C.R., Whitfield, C. Lipopolysaccharide endotoxins. Annu Rev Biochem 71 (2002) 635-700. Reed, S.G., Bertholet, S., Coler, R.N., Friede, M. New horizons in adjuvants for vaccine development. Trends Immunol 30 (2009) 23-32. 

The formation of lipid-linked sugars in vitro by Bacillus stearothermophilus. Salmonella typhimurium, and Shigella jlexneri requires an unsaturated a-isoprenyl residue in a polyprenyl phosphate, in contrast to th- t in rat-liver microsomes which requires a saturated a-isoprenyl residue. o-Glucopyranosyl polyprenyl phosphate was formed on incubation of UDP-D-[ C]glucose with particulate preparations from B. stearothermophilus, and it may serve as a D-glucosyl donor in the synthesis of an extracellular polysaccharide. The properties of the membrane-associated sialyltransferase complexes of Escherichia coli have been examined. These complexes catalyse the incorporation of iV-acetylneuraminic... 

Figure 5.1. Model of a generic LPS molecule as proposed by BCastowsky et al. The molecule is composed of the lipid A structure from E.coli, the oligosaccharide region from S. typhimurium and the O-specific side chain from Salmonella serogroup B.

Raetz, C. R. H., Purcell, S., Meyer, M. V., Qureshi, N., and Takayama, K. (1985). Isolation and characterization of eight lipid A precursors from a 3-deoxy-D-ma o-octulosonic acid-deficient mutant of Salmonella typhimurium. J. Biol. Chem. 260, 16080-16088. 

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