Lipid-binding proteins characterization

Krieg, P., Feil, S., Furstenbeegee, G., Bowden, G. T. (1993). Tumor specific overexpression of a novel keratinocyte lipid-binding protein. Identification and characterization of a cloned sequence activated during multistage carcinogenesis in mouse skin. J. Biol. Chem. 

D.A. Bernlohr, and F.B. Kraemer, Characterization of the ftinctional interaction of adipocyte lipid-binding protein with hormone-sensitive lipase, J. Biol. 

Hemoperfusion is like hemodialysis except that blood is circulated extracorporeally through a column with adsorbent material like resin or charcoal, which binds molecules electrostatically. The molecules likely to be removed are characterized as poorly dialyzable, lipid-soluble, protein bound. Among the indications for hemoperfusion in the management of poisoning include the presence of a poison in a patient with impairment of excretory system (i.e. damaged kidneys), intoxication of a drug known to produce delayed toxicity or metabolized to a more toxic metabolite (i.e. paraquat or methotrexate), deterioration of the clinical state of the poisoned patient despite conservative therapy (i.e. convulsions or cardiac arrhythmias following theophylline intoxication), or development of coma as a complication. 

Phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2) pools can be regulated by PI kinases, PtdIns(4,5)P2-phosphatases, phospholipase C (PLC), and lipid transfer and binding proteins. The contribution of each to PtdIns(4,5)P2 pools will depend on the metabolic status of the cell. Enzymes involved in PtdIns(4,5)P2 biosynthesis are shown in Figure 1. Our goal is to convince the reader of the importance of characterizing the metabolic fluxes within the discrete subcellular phospholipid microdomains that make up the lipid signaling pools. 

In the human prostate, a high-affinity folate binding protein was characterized (Holm et al. 1993), and folic acid binds to the membrane fraction that cross-reacts with the anti-prostate-specific membrane antigen (PSMA) antibody. Folate-linked nanoparticles, NP-F composed of DC-Chol as a cationic lipid. Tween 80, and folale-PEG lipid showed greater transfection efficiency in human prostate cancer LNCaP cells and human cervix carcinoma HeLa cells than in KB cells (Hattori and Maitani 2005). Folate-linked nanoparticles may bind to PMSA and then be taken up via an endocytic mechanism by LNCaP cells (Hattori and Maitani 2004). 

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