Lipid-binding assay

The aggregation and lipid-binding assays do not exhibit the same degree of selec-tivity/speciflcity as the Ab capture experiments hence these assays are useful for the examination of less complex systems, including synthetic peptides and cell culture supernatants. 

Enzyme assays—both kinetic and end-point radiocoordination of proteins, lipid assays, receptor binding assays and tissue-culture techniques... 

Berbamine was isolated from an extract of Berberis poiretii via bioassay-guided techniques using the 3H-diltiazem receptor binding assay. The alkaloid was found to possess calcium channel blocking activities (IC80 3 x 1CT6 M) and to act as an antiperoxidant as measured by the erythrocyte lipid peroxidation test. These data may provide information about the antimyocardial ischemic and antiarrhythmic effects of the berbamine [203]. 

The binding of Semliki Forest virus and its spike glycoproteins to baby hamster kidney cells has been followed by electron microscopy and quantitative binding assays. Binding of the virus to the cell involves two different modes near neutral pH the virus binds to specific glycoproteins and at low pH it binds non-specifically to the lipids of the plasma membrane. Separation of the... 

Fig. 2. Protein-lipid overlay assay to demonstrate the effect of ASAPl tyrosine phosphorylation on phospholipid binding. Protein-hpid overlay assays are performed with immobilized phosphohpids (100 pmol/spot) incubated with 0.5 tg/ml nonphosphorylated (left panel) or phosphorylated ASAPl (middle panel). Binding of Flag-ASAPl to immobilized phospholipids is detected by Western blotting with an anti-Flag antibody. Positions of the individual hpids on the array are shown on the right paneL...

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