Lipase slide test

Recently, we adapted the Ortbo Clinical Diagnostics lipase slide test [29], a dry chemistry test system used in clinical biochemistry to measure the lipase activity in human serum for the determination of the lipase activity in pancreatic extracts. The substrate, l-oleyl-2,J diacetylglyceral, and the ancillary reagents are fixed in a multilayered film (Fig, 7). Upon addition of a lipase solution, die insoluble substrate becomes emulsified... 

Figure 7 Reaction proceedings in the Ortho Clinical Diagnostics lipase slide test. l-01eyl-2,3-diacetylglycerol serves as an artificial substrate for lipase.

After the specimen has been applied to the slide, a distributor arm moves the slide to the proper incubator CM for the colorimetric and two-point rate enzyme tests (acid phosphatase, amylase, and lipase), PM for the potentiometric chemistries, and RT for the rate or kinetic incubator for the multiple-point rate enzyme chemistries. Temperature control within either the CM or RT incubator is maintained at 3 7 0.1 ° C by contact of the slide with the rotating thermal mass of the incubator. The products forming in the slides in either the CM or RT incubator are monitored at what are termed read stations by separate reflectance densitometers or reflectometers. There are, however, differences on how such measurements are made. For the enzyme slides in the CM incubator, at selected... 

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