Linear polymers steroids

This chapter describes our recent advances on the utilization of polymer-modified laccase complexes in aqueous systems towards the oxidation/polymerization of naturally hydro-phobic steroidal compounds, Equilin (EQ) and 17-P-estradiol (P-EST). We elucidate the kinetic and synthetic aspects of the process with the model compoimd 5,6,7,8-tetrahydro-2-naphthol (THN). The nano-reactor system is composed of linear poly(ethylene oxide)-dendritic poly(benzyl ether) diblock copolymer (G3-PE013k) and laccase isolated from Trametes versicolor. Other advantages of the complex in comparison to the native enzyme are its recyclability, enhanced stability, activity, and overall simplicity in product harvesting and isolation. A principle of action of the complex is suggested based on these findings and is further supported by the biphasic solid-liquid nature of the reaction medium, which exhibits continuous influx of starting material and steady solid product expulsion. Comparative experiments with linear-linear poly(styrene)-Woc -poly(ethylene oxide) copolymer under identical conditions do not evince formation of a... 

Four-element arrays, with each element consisting of three Pt electrodes (one modified 30 pm x 30 pm square working, one counter and one Pt pseudoreference), have been fabricated on glass substrates for the detection of the anabolic steroid albuterol, which is a doping concern in athletics and has been used as a food supplement for farm animals [88]. The arrays are based on working electrode modification with a molecularly imprinted polymer that is selective for albuterol over clenbuterol and terbutaline, two closely related compounds. The arrays are reusable (at least 20 times) and linear responses, as differential pulse voltammetric peak currents, for the oxidation of phenolic hydroxyl groups at about +0.45 V, occur over the 1-50 pM concentration range of albuterol. The molecularly imprinted polymer electrode films allow discrimination over similarly functionalized interferants. 

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