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Limulus polyphemus

A preferred method for the detection of pyrogens is the limulus amebocyte lysate (LAL) test. A test sample is incubated with amebocyte lysate from the blood of the horseshoe crab, Limulus polyphemus. A pyrogenic substance will cause a gel to form. This is a result of the... [Pg.415]

Development of the LAL assay was based upon the observation that the presence of Gramnegative bacteria in the vascular system of the American horseshoe crab, Limulus polyphemus, resulted in the clotting of its blood. Tests on fractionated blood showed that the factor responsible for coagulation resided within the crab s circulating blood cells, i.e. the amoebocytes. Further research revealed that the bacterial agent responsible of initiation of clot formation was endotoxin. [Pg.193]

Botton, M.L., K. Johnson, and L. Helleby. 1998. Effects of copper and zinc on embryos and larvae of the horseshoe crab, Limulus polyphemus. Arch. Environ. Contam. Toxicol. 35 25-32. [Pg.217]

ARACHNOIDS Horseshoe crab, Limulus polyphemus Molting and survival adversely affected during 5-day exposure 11... [Pg.1000]

Weis, J.S. and A. Ma. 1987. Effects of the pesticide diflubenzuron on larval horseshoe crabs, Limulus polyphemus. Bull. Environ. Contam. Toxicol. 39 224-228. [Pg.1022]

Figure 7 X-ray structure of the active site of the deoxygenated form of haemocyanin from Limulus polyphemus... Figure 7 X-ray structure of the active site of the deoxygenated form of haemocyanin from Limulus polyphemus...
Toxin assay (bactaial) amoebocyte lysate/Sigma 2000 used in d ecticm and quantitatioi of aidotoxins in plasma Cdien 1979 marine hcx-seshoe crab, Limulus polyphemus (L.), Chelicerata... [Pg.197]

Sialic acids may also be specifically stained by using peroxidase-labelled Limulus polyphemus agglutinin in combination with 3,3 -diaminobiphenyl or Aleian Blue, and the procedure was tested with various, mammalian tissues by light-microscopy.199... [Pg.174]

Strong, S.J. Ellington, W.R. Isolation and sequence analysis of the gene for arginine kinase from the chelicerate arthropod, Limulus polyphemus insights into catalytically important residues. Biochim. Biophys. Acta, 1246, 197-200 (1995)... [Pg.398]

Another remarkable example is extension of the acrosomal process from a sperm cell of the horseshoe crab Limulus polyphemus at fertilization. A bundle of actin filaments in a crystalline state lies coiled around the base of the nucleus. At fertilization the bundle uncoils and slides through a tunnel in the nucleus to form a 60 pm-long acrosomal process within a few seconds. The uncoiled bundle is also crystalline. The coiled bundle is apparently overtwisted and an actin crosslinking protein scrum mediates the conformational alteration that takes place.339 A somewhat related process maybe involved in contraction of bacteriophage tails (pp. 363,364)... [Pg.1122]

Both cations play a stabilizing role in various biological structures. Calcium ions are necessary to link the 24-subunit aggregates of the dioxygen carrier hemocyanin of Limulus polyphemus into the native 48-subunit molecule.121 The divalent character of Ca2+ probably allows it to crosslink... [Pg.564]

See other pages where Limulus polyphemus is mentioned: [Pg.372]    [Pg.186]    [Pg.407]    [Pg.604]    [Pg.677]    [Pg.691]    [Pg.193]    [Pg.211]    [Pg.399]    [Pg.451]    [Pg.227]    [Pg.172]    [Pg.130]    [Pg.218]    [Pg.218]    [Pg.173]    [Pg.186]    [Pg.407]    [Pg.604]    [Pg.677]    [Pg.691]    [Pg.1000]    [Pg.175]    [Pg.131]    [Pg.292]    [Pg.386]    [Pg.24]    [Pg.1895]   
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Limulus

Limulus polyphemus amoebocyte lysate

Limulus polyphemus lectin

Limulus polyphemus, hemocyanin

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