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Limulus amebocyte lysate

Pyrogenic A fever-producing substance. The presence of these substances is determined by the Limulus Amebocyte Lysate (LAL) test and measured in EU/ml (endotoxin units per milliliter). [Pg.623]

A preferred method for the detection of pyrogens is the limulus amebocyte lysate (LAL) test. A test sample is incubated with amebocyte lysate from the blood of the horseshoe crab, Limulus polyphemus. A pyrogenic substance will cause a gel to form. This is a result of the... [Pg.415]

Develeeshouwer, M.J., Comil, M.F. and Dony, J. (1985). Studies on the sensitivity and specificity of the limulus amebocyte lysate test and rabbit pyrogen assays. Appl. Environ. Microbiol. 50 1509-1511. [Pg.401]

Sakai H, Hisamoto S, Fukutomi I, et al. Detection of lipopolysaccharide in hemoglobin-vesicles by Limulus amebocyte lysate test with kinetic-turbidimetric gel clotting analysis and pretreatment of surfactant. J Pharm Sci 2004 93 310. [Pg.89]

Limulus amebocyte lysate is obtained from a licensed manufacturer. Each lot of reagent is tested per USP for release. Endotoxin used in all bacterial endotoxin analysis testing is obtained from a licensed manufacturer and is standardized against the USP reference standard. [Pg.533]

The determination of the endotoxin with limulus amebocyte lysate (LAL) is based on gel formation of a mixture consisting of a solution of endotoxin of Gram-negative bacteria with a solution of lysate. The extent and speed of the reaction depend on the endotoxin concentration, pLL, and temperature. The reaction requires the presence of certain cations, a proclotting enzymes system, and clottable protein, which are produced by lysate. [Pg.958]

Novitsky, TJ. (1996). Limulus amebocyte lysate (LAL) assays. In Automated Microbial Identification and Quantitation Technologies for the 2000s, W.P. Olson, ed. Inter-pharm Press, Buffalo Grove, IL, 277-298. [Pg.214]

Limulus Amebocyte Lysate test kit (Pyrogen Plus, BioWhittaker Inc., Walkersville, MD). [Pg.127]

Following the dry-heat cycle, aseptically transfer the units containing endotoxin to an aseptic area for extraction procedures, sampling, and conducting the limulus amebocyte lysate (LAL) test. [Pg.150]

Endotoxin Detection - from Limulus Amebocyte Lysate to Recombinant Factor C... [Pg.187]

Guideline on Validation of the Limulus Amebocyte Lysate Test as an End-Product... [Pg.917]

The USP now recognizes two tests—the pyrogen test conducted with rabbits and the bacterial endotoxins test, also termed the limulus amebocyte lysate... [Pg.917]

Bacterial Limulus amebocyte lysate from manufacturing operations. Gives an idea of processing... [Pg.13]

See other pages where Limulus amebocyte lysate is mentioned: [Pg.140]    [Pg.246]    [Pg.399]    [Pg.972]    [Pg.257]    [Pg.438]    [Pg.73]    [Pg.227]    [Pg.230]    [Pg.944]    [Pg.968]    [Pg.106]    [Pg.106]    [Pg.140]    [Pg.185]    [Pg.615]    [Pg.187]    [Pg.128]    [Pg.155]    [Pg.307]    [Pg.96]    [Pg.113]    [Pg.140]   
See also in sourсe #XX -- [ Pg.73 ]

See also in sourсe #XX -- [ Pg.3057 ]

See also in sourсe #XX -- [ Pg.198 ]



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