Light microscopy, image reconstruction

Schematic diagram of the procedures followed for image reconstruction in light microscopy (top) and x-ray crystallography (bottom).

Several far-field light microscopy methods have recently been developed to break the diffraction limit. These methods can be largely divided into two categories (1) techniques that employ spatially patterned illumination to sharpen the point-spread function of the microscope, such as stimulated emission depletion (STED) microscopy and related methods using other reversibly saturable optically linear fluorescent transitions (RESOLFT) [1,2], and saturated structured-illumination microscopy (SSIM) [3], and (2) a technique that is based on the localization of individual fluorescent molecules, termed Stochastic Optical Reconstruction Microscopy (STORM [4], Photo-Activated Localization Microscopy (PALM) [5], or Fluorescence Photo-Activation Localization Microscopy (FPALM) [6]. In this paper, we describe the concept of STORM microscopy and recent advances in the imaging capabilities of STORM. 

Interfacing Light Microscopy with TEM Fixation and Specimen Preparation for TEM Selective Staining of Nuclear Components Image Analysis and Enhancement Three-Dimensional Reconstructions by TEM... 

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