Ligand-binding studies azide

Obviously the investigator should also be aware of any ground state reactions peculiar to the system under study. For example 2-azidopyridines isomerize to tetrazoles which are usually less sensitive than the azide to irradiation (Fig. 3.1). The position of the equilibrium is both temperature, solvent and pH dependent. Even if the equilibrium constant for such an isomerization in solution is known the situation in a ligand binding site cannot be predicted. Azide-tetrazole isomerization was an important consideration in experiments using 2-azidoadenine derivatives which have recently been described by MacFarlane et al. (1982). 

Adjuvants such as fluoride ions, were shown to suppress the quenching effect from uric acid present in serum sample [10]. Additionally, a lanthanide complex designed for ceU-based assay should be insensitive to luminescence quenching by azide [27], which is a common internalization inhibitor used in ligand-receptor binding studies [28]. 

A majority of the host systems mentioned so far have been shown to encapsulate halide guests as a consequence of the symmetrical nature of their binding pockets, which readily adapt to the spherical symmetry of the halide ion. A study of the bis-tren macrobicyclic ligand 19-6H, however, has revealed that, in addition to accommodating halide anions, it is also able to encapsulate azide, N, within its cylindrical cavity. Solution stability constant measurements indicate that the host... 

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