Lectins specific for

It is well known that intestinal M cells have the ability to efficiently transcytose macromolecules and inert particles, which has stimulated researchers to target M cells for oral delivery of macromolecular drugs and particles.26,52 For example, Ulex europaeus 1 (UEAl), a lectin specific for a-L-fucose residues, selectively binds to mouse Peyer s patch M cells.56 It has become apparent that UEAl can be used to target macromolecules to mouse Peyer s patch M cells and to enhance macromolecular... 

Adhesion of the fungus to specific nematode species and regions of the nematode body is mediated by a fungal lectin specific for N-acetylneuraminic acid (sialic acid) (22) It has since been suggested that steric configuration of sialic acid residues on the nematode surface serve as stimuli for the processes of adhesion and infection (23). ... 

The invasion of human erythrocytes by plasmoidal merozoites is a key event during malaria infection. Sialidase treatment of the erythrocytes renders the cells resistant to invasion by different isolates of P. falciparum, suggesting that the interaction between the parasite and the erythrocytes is mediated by a plasmodial lectin specific for sialic acid [145]. Different mezoroite surface components have been proposed as the putative lectins, among them the well-defined surface antigen Pf200 [146]. Binding of the isolated... 

The E. histolytica lectin specific for chitooligosaccharides is a 220 kDa plasma membrane glycoprotein that inhibits the attachment of amoebic tropozoites to layers of cultured MDCK cells [152,153]. 

VI. Calnexin and Calreticulin Are Lectins Specific for Monoglucosylated Oligosaccharides... 

To date, monoclonal antibodies or lectins specific for almost eveiy cell-type of the nephron and collecting (duct) system are available (Figure 2). This array of monoclonals/lectins represents a powerful tool for the immunoselection of individual cell-types. However, the applicability of antibodies and lectins for immunoselection is limited by the membrane expression density of the antigens/oligosaccharide moieties sought, their stability and affinity. 

Hatton, M. W. C., Marz, L., Berry, L. R., Debaiine, M. T. and Regoeczi, E., 1979, Bland tri-antennary human transferrin glycopeptides and their affinities for the hepatic lectin specific for asialo-glycoproteins. Biochemical Journal 181, 633 - 638. 

Lidnart, Y., Gautier, C., and Domard, A. 1991. Isolation from Rubus cell-suspension cultures of a lectin specific for glucosamine oligomers. Planta 184(1) 8-13. 

Fig. 13 Glycoprofiling of placental and glycoengineered hCG based on the binding of specific lectins. Lectins specific for a2,3/6-bound sialic acids (black bars SNA, Sambucus nigra agglutinin), a2,6-bound sialic acids (grey bars PSL, Polyporus squamosus lectin) and mannose-rich oligosaccharides (white bars GNL Galanthus nivalis lectin).

Purified I-labelled lectins have been interacted with cells of the mosquito, Aedes aegyptii, and with cells of human KB tumour. Relative to human KB cells, mosquito cells bound less of those lectins specific for L-fucose and d-galactose, which are terminal residues in many mammalian glycoproteins. 

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