Lectin-resistant

Kilpatrick, D. C., Pusztai, A., Grant, G., et al. Tomato lectin resists digestion in the mammalian alimentary canal and binds to intestinal villi without deleterious effects. 

Dennis, J. W., Donaghue, T. P. and Kerbel, R. S. (1981). Membrane-associated alterations detected in poorly tumorigenic lectin-resistant variant sublines of a highly malignant and metastatic murine tumor. J. Natl. Cancer Inst. 66, 129-137. 

Finne, J., Burger, M. M. and Prieels, J. P. (1982). Enzymatic basis for a lectin-resistant phenotype increase in a fucosyltransferase in mouse melanoma cells. J. Cell Biol. 92, 277-282. 

Finne, J., Castori, S., Feizi, T. and Burger, M. M. (1989). Lectin-resistant variants and revertants of mouse melanoma cells differential expression of a fucosy-lated cell-surface antigen and altered metastasizing capacity. Int. J. Cancer 43, 300-304. 

Stanley, P, Caillibot, V. and Siminovitch, L. (1975). Selection and characterization of eight phenotypically distinct lines of lectin-resistant Chinese hamster ovary cell. Cell 6, 121-128. 

Reitman, M. L., Trowbridge, L. S., and Kornfeld, S. (1982). A lectin-resistant mouse lymphoma cell line is deficient in glucosidase II, a glycoprotein processing enzyme. J. Biol. Chem. 257, 10357-10363. 

Studies on wheat germ lectin resistant cell lines and on the effect of Swainsonine (an inhibitor of mannosidase-II) have confirmed the conclusions from this elegant experiment. 

The techniques used to obtain lectin-resistant cell lines are in themselves quite revealing. Thus far, such mutants have always been obtained from... 

Reitman, M.L., Trowbridge, I.S. Komfeld, S. (1982) A Lectin-resistant Mouse... 

Biomolecular Organisation, CIBA Foundation Symposium (G.E.W. Wolstenholme M. O Connor, eds), J. A. Churchill, London, pp. 357-417 Robertson, M.A., Etchison, J.R., Robertson, J.S. Summers, D.F. (1978) Specific Changes in the Oligosaccharide Moieties of VSV Grown in Different Lectin-resistant CHO Cells , Cell, 13, 515-26... 

The original nomenclature for the various isoforms of fucosyltransferases, based upon the order in which the genes were isolated, was established by Pamela Stanley [1] and further extended by John Lowe. FucT I and II were used to designate fucosyltransferases activities found in the lectin-resistant CHO cell lines LECH and LEC12 the next five fucosyltransferases identified were human a3,4-fucosyl-transferases whose genes have been cloned (FucT III to VII) [2-8]. 

Sasaki K, Watanabe E, Kawashima K, Sekine S, Dohi T, et al. Expression Cloning of a Novel Gal beta(l-3/l-4)GlcNAc alpha 2,3-Sialyltransferase Using Lectin Resistance Selection. J. Biol Chem. 1993 268 22782-22787. 

J.W. Dennis and S. Laferte, Co-reversion of a lectin-resistant mutation and non-metastatic phenotype in murine tumor cells. Int. J. Cancer, 38 (1986) 445-450. 

Sasaki, K., Watanabe, E., Kawashima, K., Sekine, S., Dohi, T., Oshima, M., Hanai, N., Nishi, T., and Hasegawa, M., 1993, Expression cloning of a novel Gal(3(l-3/l-4)GlcNAc a2,3-sialyltransferase using lectin resistance selection, J. Biol. Chem. 268 22782-22787. 

Dennis, J. W., Laferte, S., Fukuda, M., Dell, A., and Carver, J. P., 1986, Asn-linked oligosaccharides in lectin-resistant tumor-cell mutants with varying metastatic potential, Eur. J. Biochem. 161 359-373. 

Yogeeswaran, G., and Tao, T., 1980, Cell surface sialic acid expression of lectin-resistant variant clones of B16 melanoma with altered metastasizing potential, Biochem. Biophys. Res. Commun. 95 1452-1460. 

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