Wheat germ lectins

Fig. 6.—Elution Pattern for Wheat-germ Lectin from an Adsorbent Prepared from N,N -Diacetylchitobiose and (2-Aminoethyl)-Bio-Gel by Reductive Animation. [Initial elution with phosphate buffer in saline, followed by elution with 0.1 M 2-acetamido-2-deoxy-D-glucose in phosphate buffer and saline (arrow) (see Ref. 25).]...

Figure 2.18 Wheat germ lectin isolation using A -acetyl-o-glucosamine as the ligand. Chromatography conditions column, Waters API glass column, 100 x 10 mm I.D. flow rate, 1 ml/min detection, UV absorbance at 280 nm. The sample was 1 ml of crude wheat germ preparation 1.2 mg/ml of lectin was eluted from a crude preparation with 6.8 mg/ml total protein. (Reprinted from Ref. 64 with permission.)...

Concanavalin A, lentil lectin, wheat germ lectin... 

Lentil Lectin Sepharose Wheat Germ Lectin Lectins Membrane proteins, glycoproteins, polysaccharides Pharmacia... 

The method of lectin precipitation is based on selective precipitation of BALP with wheat germ lectin. The difference between the catalytic concentration before the precipitation and after can relatively successfully quantified in the form of the catalytic concentration of BALP (R7). 

R7. Rosalki, S. B., Bone-origin alkaline phosphatase in plasma by wheat-germ lectin methods in bone disease. Clin. Chim. Acta 226, 143-150 (1994). 

Specificity chitobiose core of N-linked oligosaccharides, [GIcNAc (I3> 1,4GlcNAc) 1-2 > IJ GIcNAc] HiTrap Wheat Germ Lectin 17-5107-01 5 x 1 ml ovomuroid 4 mg /ml... 

Specificity as listed for each column HiTrap Lectin Test Kit 17-5109-01 4 x 1 ml columns of HiTrap Con A HiTrap Lentil Lectin HiTrap Peanut Lectin HiTrap Wheat Germ Lectin as listed above... 

Cibachron blue dye, or derivatives of AMP, the NADH, or NADPH Lectins concanavalin A, lentil lectin, wheat germ lectin configurations Trypsin inhibitor, methyl esters of amino acids, or D-amino acids Phenylboronic acid... 

Two approaches have been proposed to improve the electrophoretic separation between bone and liver ALPs. Both methods exploit differences in the carbohydrate portions of the two forms of ALP. In one, electrophoresis is carried out in the presence of wheat germ lectin, which retards bone ALP migration more than the liver enzyme migration. In the other, serum is treated briefly (i.e.> for 15 min at 37 °C), with neuraminidase to remove part of the terminal sialic acid residues. As the sialic acid residues of bone ALP are more readily attacked than those of liver ALP, the electrophoretic mobility of the bone form is reduced more than that of liver ALP. The improved separation allows quantitative estimates to be made by densitometric scanning (Figure 21-5). ... 

The use of wheat germ lectin, binding the N-acetylglu-cosamine residues present in different amounts on individual isoenzymes, has also been the basis of a commercial technique for the selective precipitation and measurement of bone ALP. However, this technique does not provide sufficient accuracy and precision for quantifying the bone fraction. 

Gonchoroff DG, Branum EL, O Brien JF. Alkaline phosphatase isoenzymes of liver and bone are incompletely resolved by wheat-germ-lectin affinity chromatography. Clin Chem 1989 35 29-32. 

Mattiazzo M, Ramasamy I. Wheat germ lectin affinity electrophoresis of serum alkaline phosphatase with commercially available gels. Clin Chem 1993 39 1404-7. 

Affinity Chromatography is performed on a unique stationary phase which has a specific bio-active hgand bonded onto a solid support. It is mostly used with bio-molecules and only the active component of a sample is attracted to the stationary phase (e.g., wheat germ lectin for polysaccharides and soybean trypsin inhibitor for proteases). The remaining chemicals are washed off and... 

The 93- to 95-residue neurophysins act as carriers for vasopressin and ocytocin, forming specific complexes with them. Neurophysins contain 14 cysteine residues, which form seven disulfide bonds. There is a striking similarity in sequence between the neurophysins, snake venom toxins, a wheat germ lectin (agglutinin), a ragweed pollen allergen, and a small plant protein called hevein. On the basis of the alignment of cysteine residues, Drenth proposed that all of these proteins have a disulfide-linked core whose structure is shown in Fig. 30-16. 

Studies on wheat germ lectin resistant cell lines and on the effect of Swainsonine (an inhibitor of mannosidase-II) have confirmed the conclusions from this elegant experiment. 

Agarose Wheat Germ Lectin Agarose Wheat germ lectin 1-2mg/mL n.a n.a. Glycoproteins, polysaccharides, membrane proteins AP Biotech ... 

Dimeric wheat-germ lectin (as determined by X-ray analysis at 2.2 A resolution) consists of two closely associated protomers (each containing 164 amino-acid residues) centred about the crystallographic two-fold axis. Two carbohydratebinding sites of the lectin were identified. 

---