Leaves, paper chromatography

The leaf flavonoids of the cruciferous species such as Camelina sativa, Crambe abyssinica, Crambe hispanica, Thlaspi arvense, Brassica napus and Sinapis alba were separated and identified with the combination of HPLC, TLC and paper chromatography. Llavonoid aglycones were extracted by cutting fresh three-week-old leaves in tiny pieces and boiled in 50 ml of 2 M HC1 for 45 min. 

Roberts, E. A. H., and Wood, D. J. 1951b. The separation of polyphenols in tea leaf by paper chromatography with water as a mobile solvent. Biochem. J. 49, xxxiii. 

Absorption spectra of pigments of box eledr separated by the paper chromatography (Fig 1) indicate that chlorophyls in diferent tissues are the same, when carotenoids seam to be different. The shifts of absorbtion maxima of carotenoids and xanthophylls could be either caused by different chemical forms or by different concentrations of pigments. Similar results were obtained with pigments of Chlorophytum (Fig. 2). Further more in leaf of this plant (Fig. 3) the distinct pigment with a new absorption maxima is detected. 

Arrendale, R.F., R.F. Severson, and O.T. Chortyk The application of capillary gas chromatography to the analyses of tobacco leaf, smoke, and pyrolyzate compounds 34th Tobacco Chemists Research Conference, Program Booklet and Abstracts, Vol. 34, Paper No. 14, 1980, p. 8. 

Ethylene Release Ethylene release froM excised leaf discs during incubation in sealed flasks was quantified by gas chroMatography. The COx content of the flask headspace was Maintained by inclusion of a centrewell containing filter paper and sodiuM bicarbonate in phosphate buffer. The 00 level was varied by changing the buffer pH and the bicarbonate concentration (9). Headspace (X3 levels were Monitored by infrared gas analysis. 

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