Laser-induced fluorescence microchip capillary electrophoresis

HPLC with microchip electrophoresis. Capillary RPLC was used as the first dimension, and chip CE as the second dimension to perform fast sample transfers and separations. A valve-free gating interface was devised simply by inserting the outlet end of LC column into the cross-channel on a specially designed chip. Laser-induced fluorescence was used for detecting the FITC-labeled peptides of a BSA digest. The capillary HPLC effluents were continuously delivered every 20 s to the chip for CE separation. 

Liu, B. F., Hisamoto, H., and Terabe, S. (2003). Subsecond separation of cellular flavin coenzymes by microchip capillary electrophoresis with laser-induced fluorescence detection.. Chromatogr. A 1021, 201-207. 

Jiang, G., Attiya, S., Ocvirk, G., Lee, W.E., Harrison, D.J., Red diode laser induced fluorescence detection with a confocal microscope on a microchip for capillary electrophoresis. Biosens. Bioelectronics 2000, 14, 861-869. 

Jin LJ, Giordano BC, Landers JP. Dynamic labeling during capillary or microchip electrophoresis for laser-induced fluorescence detection of protein-SDS complexes without pre- or postcolumn labeling. Anal Chem 2001 73 4994-4999. 

Qin, J., Fung, Y, Zhu, D. and Lin, B. (2004) Native fluorescence detection of flavin derivatives by microchip capillary electrophoresis with laser-induced fluorescence intensified charge-coupled device detection. J Chrom A, 1027 (1-2), 223-229. 

Duffy, C. R, MacCraith, B., Diamond, D., O Kennedy, R., and Arriaga, E. A., Fast electrophoretic analysis of individual mitochondria using microchip capillary electrophoresis with laser induced fluorescence detection, Lah. Chip, 6, 1007-1011,2006. 

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