LAPS light-addressable potentiometric

Several other approaches for detecting nucleic acids are reported in the literature, based, for example, on the light-addressable potentiometric sensor (LAPS) (Kung et al. 1990) or on acoustic wave devices (Su et al. 1996). 

In addition to the immunochromatographic assays, another system used by the Allies during Desert Storm for the detection of biological warfare agents was the Light Addressable Potentiometric System (LAPS) produced by Molecular Devices (Sunnyvale, CA)19. The LAPS detected toxins and... 

The concept of light addressable potentiometric sensors (LAPS) was introduced in 1988 [67], LAPS is a semiconductor-based sensor with either electrolyte-insulator-semiconductor (EIS) or metal-insulator-semiconductor (MIS) structure, respectively. Figure 4.13 illustrates a schematic representation of a typical LAPS with EIS structure. A semiconductor substrate (silicone) is covered with an insulator (Si02). A sensing ion-selective layer, for instance, pH-sensitive S3N4, is deposited on top of the insulator. The whole assembly is placed in contact with the sample solution. 

FIGURE 4.13 Schematic representation of a light-addressable potentiometric sensor (LAPS). 

Y. Mourzina, Y. Ermolenko, T. Yoshinobu, Y. Vlasov, H. lwasaki, and MJ. Schoning, Anion-selective light-addressable potentiometric sensors (LAPS) for the determination of nitrate and sulphate ions. Sens Actuators, B 91, 32—38 (2003). 

T. Yoshinobu, M.J. Schoning, R. Otto, K. Furuichi, Y. Mourzina, Y. Ermolenko, and H. lwasaki, Portable light-addressable potentiometric sensor (LAPS) for multisensor applications. Sens. Actuators, B 95, 352-356 (2003). 

FIGURE 1.3 Schematic drawing of a light addressable potentiometric sensor (LAPS) for pathogen detection. (Adapted from reference Gehring et al., 1998). 

Cytosensor Microphysiometer technology has been used to detect perturbation in mammalian cells (Hafner, 2000). The system measures small changes in extracellular acidification using a light addressable potentiometric sensor. If the metabolism is interfered with, acid excretion will be affected which could be sensitively measured by LAPS. In principle, this system should be suitable for monitoring pathogen interaction with mammalian cells. 

Tu, S. 1., Uknalis, J., Gore, M., and Irwin, P. (2002). The capture of Escherichia coli 0157 H7 for light addressable potentiometric sensor (LAPS) using two different types of magnetic beads. /. Rapid Methods Automat. Microbiol. 10,185-195. 

Light-addressable potentiometric sensors (LAPS) recent trends and applications... 

M. Sartore, M. Adami, C. Nicolini, L. Bousse, S. Mostarshed and D. Hafeman, Minority carrier diffusion length effects on light-addressable potentiometric sensor (LAPS) devices, Sens. Actuators A Phys., 32(1-3) (1992) 431-436. 

W. Parak, M. George, J. Domke, M. Radmacher, J. Behrends, M. Denyer, and H. Gaub, Can the light-addressable potentiometric sensor (LAPS) detect extracellular potentials of cardiac myocytes IEEE Trans. Bio-med. Eng., 47 (2000) 1106-1113. 

B. Stein, M. George, H.E. Gaub and W.J. Parak, Extracellular measurements of averaged ionic currents with the light-addressable potentiometric sensor (LAPS), Sens. Actuators B Chem., 98(2-3) (2004) 299-304. 

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