Langmuir binding

Figure 2.7 One-site Langmuir binding isotherms of propranolol to MIP and CP beads (0 25-50 pm). Data points are the mean values of three replicates and standard deviations are indicated with error bars (data...

From the above discussion, it is apparent that two-site binding models are too simple to accurately characterize most heterogeneous MIPs. Higher order Langmuir binding models have also been applied to MIPs such as tri- and tetra-Langmuir isotherms [9]. However, all of these models assume that MIPs contain a specific number of different types of sites, which is unlikely given the sources of the structural diversity of... 

Langmuir binding does not reach a plateau until very large values of x (compare Figure 27.6, in which the concentration of ligand is hundreds of pM to ensure saturation). The preferred method for determining a binding constant is to linearize the plot with Equations (27.13) or (28.20). 

K = CsICm is the partition coefficient of the solute from the mobile phase into the stationary phase (see Equation (16.39)). = VslV, is a property of the chromatography apparatus called the phase ratio. Langmuir binding is reflected in the expression fs = 1 - fm = K/(1 + K). One purpose of chromatography experiments is to measure K. Substituting Equation (27.20) into Equation (27.19) gives the average How velocity of the solute,... 

Fig. 7 Anti-MK aptamer and its therapeutic potential, (a) M-fold predicted secondary structures of three representative MKapt sequences, (b) SensOTgram of MKapt (49-nucleotide derivative) binding MK. MK was iimnobilized on a CM4 sensor chip and the RNA aptamers were injected for the indicated period. The was estimated to be 0.9 nM using global fitting curves (1 1 Langmuir binding) with four different RNA concentrations (0.05, 0.1, 0.2, and 0.4 mM). (c) Clinical scores for wild-type EAE mice administered phosphate buffered saline (circles) (n = 5) or 1 mg/kg (n = 5) MKapt (38-nucleotide derivative) (triangles) after EAE onset (14 days after immunization). P

Bloor D M, Wan-Yunis W M Z, Wan-Badhi W A, Li Y, Hoizwarth J F and Wyn-Jones E 1995 Equilibrium and kinetio studies assooiated with the binding of sodium dodeoyl sulfate to the polymers poly(propylene oxide) and ethyl-(hydroxyethyl)oellulose Langmuir 3395-400... 

The Langmuir adsorption isotherm for radioligand binding [A ] to a receptor to form a radioligand-receptor complex [A R] can be rewritten in terms of one where it is not assumed that receptor binding produces a negligible effect on the free concentration of ligand ([A free]) ... 

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