Lactic dehydrogenase and

The resulting pyruvate may be measured by coupling the reaction with lactic dehydrogenase and NADH, or chemically by using dini-trophenylhydrazine. The kinetic method has been revised by Henry and co-workers to optimize the analytical conditions, and still is the method of choice (28). 

Hemolytic anemias tend to be normocytic and normochromic and to have increased levels of reticulocytes, lactic dehydrogenase, and indirect bilirubin. 

Abston PA, Yarbrough JD. 1974. The in vivo effects of dietary mirex on hepatic lactic dehydrogenase and glutamic oxaloacetic transaminase levels in the rat. J Agric Food Chem 22 66-68. 

Enzymes activities are particularly sensitive to the anticoagulant used in collecting the specimen. Heparin inhibits acid phosphatase (W16) and muramidase (Z5). Amylase activity is inhibited by oxalate or citrate (MIO), and lactic dehydrogenase and acid phosphatase lose activity in oxalate (C2). Alkaline phosphatase is stable in oxalate, oxalate-fluoride, or heparin, but 25 mAf citrate inhibits 50% of the activity, and as little as 50 mlf EDTA is completely inhibitory (B19). Leucine aminopeptidase is inhibited by EDTA, as is creatine phosphokinase (F3). Amylase activity has been reported to be only 83% of that in serum when oxalate or citrate-plasma is used (MIO). Heparin plasma appears to have no inhibitory effect. Despite the fact that clotting factor V is not stable in oxalate or EDTA, these are often used as anticoagulants to obtain plasma for prothrombin determinations (Z2, Z4). 

Schapira, F., Dreyfus, J. C., and Schapira, G., Fetal-like patterns of lactic dehydrogenase and aldolase isoenzymes in some pathological conditions. Emymol. Biol. Clin. 7, 98-108 (1966). 

An elderly man who had been taking allopurinol 300 mg daily for 12 years developed fever and marked increases in his serum levels of lactic dehydrogenase and alkaline phosphatase within a day of starting to take tamoxifen 10 mg twice daily. He rapidly recovered when the allopurinol was stopped. The reasons for the reaction are not understood, but the authors suggested that the increased hepatotoxic effect may have resulted from tamoxifen inhibiting allopurinol metabolism, thereby increasing the serum levels of allopurinol and its metabolite. The general importance of this isolated report is not known. 

Grisiola S, Rubio V, Eeijoo B et al (1975) Inhibition of lactic dehydrogenase and of pyruvate kinase by low concentrations of quercetin. Physiol Chem Phys 7 473 75... 

If this reaction is coupled with lactic dehydrogenase and reduced DPN, the pyruvate formed will be immediately reduced to lactate, with a corresponding oxidation of the reduced DPN. The oxidation of DPNH2 is then a direct measure of the ADP present in the original solution. ... 

This hypothesis is supported by some in vitro studies in which cultured hepatocytes incubated with cheno have been shown to release lactic dehydrogenase and transaminases into the culture medium (11). The "direct toxicity against liver membrane was not observed when urso was tested using the same experimental design. This experimental evidence is of particular interest since it reflects the clinical observation that cheno in some cases produces hypertransaminasemia, but urso does not. 

Exposure of leukemia P388 to act-D causes respiratory depression, a decrease in malic and lactic dehydrogenase and an Increase in glucose-6-phosphate dehydrogenase. These effects are similar to those caused by nitrogen mustard.93 Act-D stimulates isoperoxidase activity at low concentration and represses it at higher concentration. It inhibits the activity of DNA polymerase.95... 

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