Lactate dehydrogenase 1946 Volume

Fig. 9. Simultaneous plots of reaction rate (relative Vmax) and activation volume (AV() for the lactate dehydrogenase system as functions of salt (KSCN) concentration. This mirror image may be explained by the similarity between Eq. (134) and Eq. (136). (By courtesy of Prof. G. N. Somero, unpublished.)...

The breakdown of fat leads to a transient increase in body water. Normally, however, an osmotic diuresis soon reduces the blood volume even though glomerular filtration may be impaired, as demonstrated by increased serum creatinine concentration and reduced creatinine clearance. Hepatic blood supply may also be reduced. BSP retention is increased, and the serum bilirubin rises the unconjugated bilirubin more than doubles within 48 hours. Increased serum activities of aspartate and alanine aminotransferase and of lactate dehydrogenase are observed. The enzyme changes may be linked more to focal necrosis of the liver than to general circulatory impairment. 

Boyer, P. D., ed. The Enzymes,Yoh. 5-9. New York Academic Press, 1972. [A standard reference with review articles on the glycolytic enzymes lactate dehydrogenase and alcohol dehydrogenase appear in Volume 10.]... 

The cytotoxicity tests were performed with the use of the direct contact method on the extracts obtained by an 8-d incubation of the polymer PSU and PSU/Ag composite samples, placed at the bottom of the well of a 24-well culture plate, in 2 ml of culture mediiun. The incubation was conducted at 37°C in air atmosphere with a 5% content of C02and 100% of relative air humidity. Next, 0.2 ml of ihe obtained extract and its fourfold dilution in a proper culture medium was dosed for the cultures of human osteoblasts (HTB-85 cell line, ATCC, USA) and human fibroblasts (CRL-7422 cell line, ATCC, USA) adhered to the bottom of the well of a 96-well culture plate. In the case of the control test, the extract of the examined samples was replaced by the proper volume of culture medium. The plates were incubated at 37°C in air atmosphere with a 5% content of CO and 100% of relative air humidity. The incubation time for two parallelly conducted experiments was 24 h and 48 h. The cytotoxicity of PSU and the PSU/Ag composites was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium (MTT) assay [9, 10] and by lactate dehydrogenase (LDH) activity measured with the use of a commercial cytotoxicity assay kit (Roche Diagnostics GmbH, Mannheim, Germany), [11]. Each of the indications was repeated three times. 

Hexasulfohut)dated Ceo, a free radical remover, at dosages of 10 and 100 pg/kg, reduced the total volume of infarction in both pre-treatment and treatment groups of male Long-Evans rats with focal cerebral ischaemia induced by middle cerebral artery occlusion (Huang et al. 2001). After administration of the drug, the NO content in plasma was increased and the lactate dehydrogenase (EC 1.1.1.27) levels were decreased. 

An effect of hydrostatic pressure on oligomeric proteins is the dissociation into monomers which decreases the volume. Dissociation into monomers occurs at lower hydrostatic pressure (between 0.1 and 2 kbar). It was observed in such systems as actin or tubulin. The effect of hydrostatic pressure on dissociation was investigated in detail with enolase (Weber 1979, Paladini and Weber, 1981) and with lactate dehydrogenase (Schmid et al., 1975,1978 ... 

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