Laboratory evaluation amino acid analysis

ABRF-96SEQ offered a unique opportunity for participating laboratories ability to interpret the same data. There were 95 participants in the study, of which 98% perform sequence analysis, 54% use mass spectrometty routinely, and 64% provide an internal sequence analysis service. The total number of sequence reviewers were 157 or 1.65 per response and the total years of experience were 1061.4 or 6.8 years per reviewer. Concerning everyday sequence calling, 37% report only one person reviews the data while only 17% use more than 1 person 72% say that a simple sequence is called by one person and that complex data is evaluated by more than one reviewer. There were 165 protein sequencers in the responding laboratories and are distributed as follows 133 Applied Biosystems/Perkin Elmer, 22 Hewlett-Packard, 8 Porton/Beckman, and 2 MiUigen. Respondents reported the most difficult amino acids to identify were Cys (64.5%), Trp (55%), and Ser (22.5%). 

The first part of the book examines the fundamental principles of chirality and TLC. It describes the necessary materials, laboratory equipment, procedures, and strategies for the separation, quantification, isolation, and analysis of chiral compounds. The second part evaluates the reaUworld enantioseparations and densitometric analyses. Emphasizing pharmaceutical applications, the book examines the chiral separation mechanisms and methods for analyzing the chiral purity of diastereoisomers, amino acids, beta-blockers, and NSAIDS. Topics also include commercial stationary phases and chiral modifiers of mobile phases. 

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