Labeling efficiency factors affecting

Accuracy in diagnosis depends largely on the labeling efficiency (LE) of blood cells, which can be affected by many factors. The specificity of the labeling, maintenance of viability, and normal physiological function of the labeled cells, together with sterility, apyr-ogenicity and radiopharmaceutical purity, and stability have to be carefully controlled. 

The quantum efficiencies and photostabilities of most intrinsically fluorescent biomolecules are modest relative to those of the available fluorescent labels. There are, however, several factors that can affect these quantum yields. The ionic strength and pH of the separation conditions, as well as the presence or absence of organic additives, can have a significant effect on the fluorescent properties (i.e., quantum yields and Stokes shift) of these analytes." Thus, if sensitivity is of... 

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