L-sorbose dehydrogenase

Zhao, S.G., Yao, L.M., Su, C.X., Wang, T. et al (2008) Purification and properties of a new L-sorbose dehydrogenase accelerative protein from Bacillus megaterium bred by ion-beam implantation. Plasma ScL Technol, 10, 398-402. 

Sugisawa T, Hoshino T, Nomura S, Fujiwara A (1991) Isolation and characterization of membrane-boimd L-sorbose dehydrogenase from Gluconobacter melanogenus UVIO. Agric Biol Chem 55(2) 363-370... 

L-Sorbose + NADP+ = 5-dehydro-D-fmctose + NADPH (reaction of sorbose dehydrogenase, EC 1.1.1.123)... 

This enzyme [EC 1.1.1.14], also known as L-iditol 2-dehydrogenase and polyol dehydrogenase, catalyzes the reaction of L-iditol with NAD+ to produce L-sorbose and NADH. The enzyme will also convert D-glucitol to D-fructose. 

Oxidation of secondary or primary alcohols by dehydrogenases is usually not performed biocatalytically. The reaction destroys a stereocentre, it is thermodynamically not favoured and product inhibition is a problem. It is attractive only in cases where it is necessary to discern between several hydroxy groups in a molecule. Microbial oxidation of D-glucitol to yield L-sorbose is the key step in production of vitamin C (Reichstein and Griissner, 1934). 

EC 1.1.1.14 L-Iditol dehydrogenase EC 1.1.1.27 Lactate dehydrogenase L-Iditol + NAD + t L-sorbose + NADH Lactic acid + NAD+ pyruvic acid + NADH + H + — SM... 

Polyol dehydrogenase. Sorbitol dehydrogenase. L-iditol + NAD(+) = L-sorbose + NADH. 

Y, et al. (1997) Cloning of genes coding for L-sorbose and L-sorbosone dehydrogenases from Gluconobacter oxydans and microbial production of 2-keto-gulonate, a precursor of L-ascorbic acid, in a recombinant G. oxydans strain. Appl. Environ. Microbiol, 63, 454-460. 

Gao, L.L., Du, G, Zhou, J., Chen, J. et al. (2013) Characterization of a group of pyrroloquinoline quinone-dependent dehydrogenases that are involved in the conversion of L-sorbose to 2-keto-L-gulonic acid in Ketogulonicigenium vulgare WSH-001. Biotechnol Progr, 29, 1398-1404. 

Y. et al (1997) Cloning of genes coding for L-sorbose and L-sorbosone dehydrogenases from Gluconobacter oxydans and microbial production... 

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