Kluyveromyces fragilis enzyme

GrootWassink, J.W.D. and Fleming, S.E., Non-specific P-fructofuranosidase (inulase) from Kluyveromyces fragilis batch and continuous fermentation, simple recovery method and some industrial properties, Enzyme Microb. Technol., 2, 45, 1980. 

Arnold (51) partially purified such an enzyme from cell-free extracts of bakers yeast Matile et al, (45) and Cortat et al. (44) demonstrated the existence of glucanase-containing vesicles within the cytoplasm of Sac-charomyces cerevisiae. These vesicles contained exo- as well as endo-glucanases but the enzymes were not studied in detail. Fleet and Phaff (47) obtained qualitative evidence for the occurrence of endo-/ -( 1 — 3) glucanases in the cell walls of Saccharomyces rosei, Kluyveromyces fragilis, Hansenula anomala, Pichia pastoris, and Candida utilis. K. fragilis and H. anomala contained only exo-glucanase in cell extracts (38). 

Partly purified /3-D-fructofuranosidase of a strain of Kluyveromyces Saccharomyces) fragilis hydrolyzes sucrose and raffinose with a pH optimum of 4.2, but of 5.2 for inulin.704 Furthermore, the hydrolysis of sucrose was found to be competitively inhibited by raffinose, but not by inulin, so two enzymes may be responsible for the /3-D-fruc-tofuranosidase activity of this yeast. 

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