Kinetic capillary electrophoresis

Affinity methods play a crucial role in modem life sciences. In addition to affinity purification, then-applications include quantitative analyses of biomolecules, studies of biomolecular interactions, and selection of affinity probes and dmg candidates from complex mixtures, such as combinatorial libraries. Conceptually, all affinity methods are based on noncovalent binding of a ligand (L) and a target (T) with the formation of a ligand-target complex (C)  

Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques 

Separation-based affinity methods can also be classified as kinetic or nonkinetic. Kinetic methods are those that do not assume equilibrium in reaction 1 and can thus be used for (1) quantitative affinity 

In general, homogeneous methods are preferable due to their simplicity and kinetic methods are preferable due to their enabling kinetic features. Until recently, the only mixture-based heterogeneous method with comprehensive kinetic capabilities was SPR. In this chapter, kinetic capillary electrophoresis (KCE) is described as a conceptual platform for separation-based homogeneous methods with comprehensive kinetic capabilities, which will find multiple applications in chemistry, biology, medicine, and drug discovery. 

Kinetic capillary electrophoresis is defined as capillary electrophoretic separation of species that interact during electrophoresis. Thus, KCE involves two major processes affinity interaction of L and T, described by Equation 11.1, and separation of L, T, and C based on differences in their electrophoretic velocities, vl, vy, and vc, respectively. These two processes are described by the following general system of partial differential equations  

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Drabovich, A. P., Berezovski, M., Okhonin, V., Krylov, S. N. (2006). Selection of smart aptamers by methods of kinetic capillary electrophoresis. Anal Chem 78, 3171-3178. 

KINETIC CAPILLARY ELECTROPHORESIS FOR SELECTION, CHARACTERIZATION, AND ANALYTICAL UTILIZATION OF APTAMERS... 

Kinetic capillary electrophoresis (KCE) was recently introduced as a conceptual platform for homogeneous kinetic affinity methods (Petrov et al., 2005). KCE is defined as CE separation of species that interact with each other during then-electrophoretic separation in CE. As a brief introduction to CE, the schematic of a CE instrument is depicted in Figure 9.1. 

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