Keto acids, detection paper chromatography

The detection of microgram quantities of pyrethrins, cinerins, keto alcohols, and chrysanthemum acids by paper chromatography and by application of these techniques to a study of possible metabolites enabled certain tentative conclusions that imply hydrolysis in insects of a large portion of the radioactive pyrethrins and synergists to corresponding keto alcohols and chrysanthemum acids. 

A stoichiometric amount (0.1 mM) each of enzyme-bound FAD and C-u-L-lysine (u = uniformly labelled) was incubated anaerobically until FAD was fully reduced. After deproteinization the reaction mixture was subjected to high voltage paper electrophoresis and paper chromatography. Most of the radioactivity appeared at the area corresponding to piperidine 2-carboxylic acid (a-keto-c-aminocaproic acid) (Figure 7), and a significant amount of carbon dioxide was not detected. When the reaction mixture was aerated to reoxidize FAD and then deproteinized, the radioactivity was also found at the position of piperidine 2-carboxylic acid. 

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