Isotopomer analysis

Chen, C., Krausz, K. Idle, J. and Gonzalez, F.J. (2008) Identification of novel toxicity-assodated metabolites by metabolomics and mass isotopomer analysis of acetaminophen metabolism in wild-type and Cyp2el-null mice. Journal of Biological Chemistry, 238 (8), 4543 559. 

As a result of metabolic reactions an isotope may appear at more than one position in a product, yielding two or more isotope isomers or isotopomers. These are seen individually by NMR spectroscopy and the concentration and isotope labeling patterns of the labeled compounds can be followed over a period of time. The use of this isotopomer analysis in studies of the citric acid cycle is illustrated in Box 17-C and its use in studies of glucose metabolism is considered in Chapter 17, Section L. 

In 2003, Burgess and co-workers158 reported a non-invasive means of evaluating liver function through the use of 2H- and 13C-NMR isotopomer analysis of human. 

PWN Lee, LO Byerley, EA Bergner. Mass isotopomer analysis Theoretical and practical considerations. Biol Mass Spectrom 20 451, 199E... 

Nuclear Magnetic Resonance, Isotopomer Analysis, and Modeling of Metabolism The Fasting State Lipogenesis... 

Isotopomer analysis can also be conducted by mass spectroscopy, which is more sensitive than NMR, using or labeling.) Making use... 

DNA technology might improve lysine yield in C. glutamicum. Therefore, it was of prime interest to quantitate the in vivo flux through each of the enzyme reactions potentially involved in the C3-C4 interconversion in C. glutamicum in order to decide which enzyme is responsible. For this purpose, a completely new labelling strategy based on isotopomer analysis was developed. 

Detailed Flux Information from Isotopomer Analysis... 

Fig. 7. Rationale for the detailed quantification of C3-C4 interconversion in C. glutamicum by isotopomer analysis explained in the text. The substrate mixture consists of unlabelled and uniformly labelled glucose, and [3- C]lactate as a co-substrate. Full circles/boxes represent carbons, empty ones C. Isotopomers crucial for enzyme activity identification are in...

R 505 D. Seebach, A.K. Beck and DJ. Bierbaum, The World of (3- and y-Peptides Comprised of Homologated Proteinogenic Amino Acids and Other Components , Chem. Bio divers., 2004,1,1111 R 506 D.S. Sem Chemical Proteomics from a Nuclear Magnetic Resonance Spectroscopy Perspective , Expert Rev.Proteom., 2004,1,165 R 507 A.D. Sherry, F.M.H. Jeffrey and C.R. Malloy, Analytical Solutions for C Isotopomer Analysis of Complex Metabolic Conditions Substrate Oxidation, Multiple Pyruvate Cycles, and Gluconeogenesis , Me-tab.Eng., 2004,6,12... 

Isotopomer Analysis of Glntamate An NMR Method to Prohe Metabolic Pathways Intersecting in the Citric Acid Cycle... 

Berthold, B.K., L.J. Wykes, F. Jahoor, RD. Klein and R.J. Reeds, 1994. The use of uniformly labelled substrates and mass isotopomer analysis to study intermediary metabolism. Proc. Nutr. Soc. 53, 345-354. 

Brunengraber, H., J.K. Kelleher and C. Des Rosiers, 1997. Applications of mass isotopomer analysis to nutrition research. Annu. Rev. Nutr. 17, 559-596. 

Chatham, J.C., B. Bouchard and C. Des Rosiers, 2003. A comparison between NMR and GCMS C-isotopomer analysis in cardiac metabolism. Mol. Cell. Biochem. 249, 105-112. 

Des Rosiers, C., L. Di Donato, B. Comte, A. Laplante, C. Marcoux, F. David, C. A Fernandez and H. Bmnengraber, 1995. Isotopomer analysis of citric acid cycle and gluconeogenesis in rat liver. Reversibility of isocitrate dehydrogenase and involvement of ATP-citrate lyase in gluconeogenesis. J. Biol. Chem. 270, 10027-10036. 

Lee, W.N., S. Sorou and E. A Beigner. 1991. Glucose isotope, carbon recycling, and gluconeogenesis using [U- C] glucose and mass isotopomer analysis. Biochem. Med. Metab. Biol. 45, 298-309. 

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