Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Isotherm frontal analysis

Note that knowledge of the initial slopes of the adsorption isotherms gives some constraint to be fullfilled between parameters X, N, and K. In order to fit the adsorption isotherms, frontal analysis has performed with the pure components at 1, 25, 50, 75 and 100 g on the analytical column at 1 ml min k... [Pg.269]

Other gas-chromatographic techniques besides frontal analysis were also utilized at finite concentration to determine the adsorption isotherms frontal analysis through characteristic points [145], elution through characteristic points [146—148] and, elutipn on a plateau [149, 150]. [Pg.152]

Experiments Sorption equihbria are measured using apparatuses and methods classified as volumetric, gravimetric, flow-through (frontal analysis), and chromatographic. Apparatuses are discussed by Yang (gen. refs.). Heats of adsorption can be determined from isotherms measured at different temperatures or measured independently by calorimetric methods. [Pg.1504]

For determining the adsorption isotherm, the equilibrium concentrations of bound and free template must be reliably measured within a large concentration interval. Since the binding sites are part of a solid, this experiment is relatively simple and can be carried out in a batch equilibrium rebinding experiment or by frontal analysis. [Pg.163]

The precise measurement of competitive adsorption isotherms not only of theoretical importance but may help the optimization of chromatographic processes in both analytical and preparative separation modes. The methods applied for the experimental determination of such isotherms have been recently reviewed [90], Frontal analysis using various flow rates can be successfully applied for the determination of competitive adsorption isotherms [91]. [Pg.37]

According to the equilibrium dispersive model and adsorption isotherm models the equilibrium data and isotherm model parameters can be calculated and compared with experimental data. It was found that frontal analysis is an effective technique for the study of multicomponent adsorption equilibria [92], As has been previously mentioned, pure pigments and dyes are generally not necessary, therefore, frontal analysis and preparative RP-HPLC techniques have not been frequently applied in their analysis. [Pg.38]

O. Lisec, P. Hugo and A. Seidel-Morgenstein, Frontal analysis method to determine competitive adsorption isotherms. J. Chromatogr.A 908 (2001) 19-34. [Pg.60]

Using this methodology via measurement of adsorption isotherms, Guiochon and coworkers investigated site-selectively the thermodynamics of TFAE [51] and 3CPP [54] on a tBuCQD-CSP under NP conditions using the pulse method [51], the inverse method with the equilibrium-dispersive model [51, 54], and frontal analysis [54]. [Pg.45]

Fig. 6.1 A breakthrough curve generated by the frontal analysis method [31], The analysis represents a high-volume injection of caffeine through a reversed-phase column, at a concentration representative of the linear region of the binding isotherm. Adapted with permission from Elsevier. Fig. 6.1 A breakthrough curve generated by the frontal analysis method [31], The analysis represents a high-volume injection of caffeine through a reversed-phase column, at a concentration representative of the linear region of the binding isotherm. Adapted with permission from Elsevier.
Calculation of the isotherm can be done by the method of Cremer and Huber [7] for pulse measurements or by the approach of James and Phillips [8] for frontal analysis. [Pg.634]

Frontal analysis brings with it the requirement of the system to have convex isotherms (see Section 1.2.6). This results in the peaks having sharp fronts and well-formed steps. An inspection of Figure 1.3 reflects the problem of analytical frontal analysis— it is difficult to calculate initial concentrations in the sample. One can, however, determine the number of components present in the sample. If the isotherms are linear, the zones may be diffuse. This may be caused by three important processes inhomogeneity of the packing, large diffusion effects, and nonattainment of sorption equilibrium. [Pg.6]

As with frontal analysis, displacement analysis requires convex isotherms. Once equilibrium conditions have been attained, an increase in column length serves no useful purpose in this technique because the separation is more dependent on equilibrium conditions than on the size of column. [Pg.7]

SUMMARY. The frontal technique does not lend itself to many analytical applications because of the overlap of the bands and the requirement of a large amount of sample. However, it may be used to study phase equilibria (isotherms) and for preparative separations. (Many of the industrial chromatographic techniques use frontal analysis.) Displacement development has applications for analytical LC (e.g. it may be used as an initial concentrating step in GC for trace analysis). This technique may also be used in preparative work. The outstanding disadvantage of both of these techniques is that the column still contains sample at the conclusion of the separation. Thus, regeneration of the column is necessary before it can be used again. [Pg.8]

Computer-aided frontal analysis chromatography can be used to determine surface areas and adsorption isotherms (4). Both permanent gases and adsorbates that are liquids above room temperature could be used in this system. [Pg.557]

There is a fundamental relationship described in chromatographic theory between the retention volume of a elution peak and the mid-point of a breakthrough curve achieved by operating the column under frontal analysis conditions (41 ). In the Henry s Law region of the adsorption isotherm, the net retention volume and its measurement can be used to describe the variation of sorbate breakthrough volume as illustrated in Figure 8. Utilizing the experimental apparatus described in the last section, retention volumes were measured as a function of pressure at 40°C (T =... [Pg.161]

Direct determination of the column saturation capacity requires measurement of the adsorption isotherm. Use of methods such as frontal analysis, elution by characteristic point are classical techniques. Frontal analysis and elution by characteri.stic point require mg or gram quantities of pure product component. It is also possible to estimate the column saturation capacity from single-component overloaded elution profiles using the retention time method or using an iterative numerical method from a binary mixture [66J. [Pg.242]

Fig. 5.8. Frontal analysis of the binding of D- and L-PA to an MIP imprinted with L-PA. The MIP was prepared using dichloromethane as diluent following the procedure shown in Fig. 5.2. Fitting (lines) of the experimental isotherm data (symbols) for L-PA (A) and D-PA (B) to the bi-Langmuir model (main figure), the Langmuir model (left inset) and the Freundlich model (right inset). For the runs at 40°C solid lines and plus symbols, at 50°C long dashed lines and crosses, at 60°C short dashed lines and stars, at 70°C dotted lines and squares. Mobile phase MeCN/potassium phosphate buffer 0.05 M, pH 5.85 70/30 (v/ v). From Sajonz et al. [40]. Fig. 5.8. Frontal analysis of the binding of D- and L-PA to an MIP imprinted with L-PA. The MIP was prepared using dichloromethane as diluent following the procedure shown in Fig. 5.2. Fitting (lines) of the experimental isotherm data (symbols) for L-PA (A) and D-PA (B) to the bi-Langmuir model (main figure), the Langmuir model (left inset) and the Freundlich model (right inset). For the runs at 40°C solid lines and plus symbols, at 50°C long dashed lines and crosses, at 60°C short dashed lines and stars, at 70°C dotted lines and squares. Mobile phase MeCN/potassium phosphate buffer 0.05 M, pH 5.85 70/30 (v/ v). From Sajonz et al. [40].
One of the most important applications of frontal chromatography is the determination of equilibrium adsorption isotherms. It was introduced for this purpose by Shay and Szekely and by James and Phillips [4,5], The simplicity as well as the accuracy and precision of this method are reasons why the method is so popular today and why it is often preferred over other chromatographic methods e.g., elution by characteristic points (ECP) or frontal analysis by characteristic points (FACP) [6,7]. Frontal chromatography as a tool... [Pg.722]

It has to be noted that the half-height and inflection-point methods do not give reliable results if the isotherm is concave upward and ascending concentration steps are performed. The same is true for a convex upward isotherm and descending concentration steps. The reason for this is that, in these cases, a diffuse breakthrough profile is obtained and, consequently, errors are made in the accurate determination of the retention volumes when they are derived from the half-height or the inflection point. The diffuse profile can, however, be used for the determination of isotherms by the frontal analysis by characteristic points method (FACT). [Pg.723]

There are two possibilities for performing a frontal chromatography experiment for the purpose of the determination of equilibrium isotherms. The step-series method uses a series of steps starting from C = 0 to C +i. After each experiment, the column has to be reequilibrated and a new step injection with a different end concentration C +i can be performed. In the staircase method, a series of steps is performed in a single run with concentration steps from 0 to Q, Q to C2,.. ., C to C +i. The column does not have to be reequilibrated after each step and, therefore, the staircase method is faster than the step-series method. Both modes of frontal analysis give very accurate isotherm results. [Pg.723]

Nonlinearity of the Langmuir adsorption isotherms is observed even in noncompetitive chromatographic processes. Individual adsorption isotherms can be found experimentally using frontal analysis at overload conditions however, the adsorption isotherms in the separation of mixtures are different because of the interference of other compounds in the mixture. In PHPLC method development, it is necessary to optimize separation conditions and column loading experimentally. [Pg.1260]

The dynamic methods are based on direct chromatography and are popular because they are faster and easier to automate. Four direct chromatographic methods that are available for determination of adsorption isotherms are frontal analysis (FA) [13, 109] frontal analysis by characteristic points (FACP) [109], elution by characteristic points (ECP) [109] and the perturbation peak (PP) method [118-121], The FACP and ECP methods have... [Pg.53]

Figure 9. The principle of the frontal analysis technique. In the uper part of the figure a theoretic staircase is shown with 10 steps, showing the increment of solute in the stationary phase. A new step starts every 40 mL and is shown in the figure by a vertical line. In the lower part of the figure the corresponding isotherm is shown. The following values have been used VT = 2 mL, a = 120, b = 0.4 mM 1. Vs = 0.4 mL. The illustration was used with kind permission from Gustaf Gotmar [111]. Figure 9. The principle of the frontal analysis technique. In the uper part of the figure a theoretic staircase is shown with 10 steps, showing the increment of solute in the stationary phase. A new step starts every 40 mL and is shown in the figure by a vertical line. In the lower part of the figure the corresponding isotherm is shown. The following values have been used VT = 2 mL, a = 120, b = 0.4 mM 1. Vs = 0.4 mL. The illustration was used with kind permission from Gustaf Gotmar [111].
The accurate determination of the adsorption isotherm parameters of the two enantiomers on a CSP is of fundamental importance to do computer-assisted optimization to scale up the process. Such determinations are usually done with an analytical column and the most traditional method to determine the parameters and saturation capacity is by frontal analysis (see section 3.4.2). The aim of paper III was to investigate the adsorption behavior and the chiral capacity of the newly developed Kromasil CHI-TBB column using a typical model compound. Many of the previous studies from the group have been made on low-capacity protein columns which has revealed interesting information about the separation mechanism [103, 110, 111], For this reason a column really aimed for preparative chiral separations was chosen for investigation [134], As solute the enantiomers of 2-phenylbutyric acid was chosen. [Pg.66]

The isotherm data acquired from frontal analysis over a broad concentration range fitted well to the bi-Langmuir model, see Figure 18, demonstrating that the adsorption on Kromasil CHI-TBB is heterogeneous with two types of sites. The saturation capacity of site II obtained from the bi-Langmuir isotherm parameters were qs>n = 130 mM for (R)-(-)-2-phenylbutyric acid and qs,n= 123 mM for (S)-(+)-2-phenylbutyric. [Pg.67]

Figure 18. Isotherms for (R) and (S)-2-phenylbutyric acid, experimentally acquired by frontal analysis and compared with their fits with bi-Langmuir adsorption isotherm parameters. The lines are calculated data using the best single bi-Langmuir isotherm parameters. Figure 18. Isotherms for (R) and (S)-2-phenylbutyric acid, experimentally acquired by frontal analysis and compared with their fits with bi-Langmuir adsorption isotherm parameters. The lines are calculated data using the best single bi-Langmuir isotherm parameters.
Figure 3. Sorption Isotherm of Starch Determined at 25°C by Modified Frontal Analysis and Frontal Method. Figure 3. Sorption Isotherm of Starch Determined at 25°C by Modified Frontal Analysis and Frontal Method.

See other pages where Isotherm frontal analysis is mentioned: [Pg.163]    [Pg.133]    [Pg.175]    [Pg.278]    [Pg.218]    [Pg.298]    [Pg.185]    [Pg.947]    [Pg.199]    [Pg.66]    [Pg.129]    [Pg.162]    [Pg.723]   
See also in sourсe #XX -- [ Pg.274 , Pg.278 , Pg.286 ]




SEARCH



Adsorption isotherms frontal analysis

Frontal

Frontal Analysis isotherm measurements

Frontal analysis

Isotherm analysis

Isothermal analysis

© 2024 chempedia.info