Isomerases glucose-6-phosphate isomerase

PHOSPHOGLUCOSE ISOMERASE Glucose-6-phosphate isomerase, PHOSPHOGLUCOISOMERASE D-Glucose 6-phosphate synthesis, 6-PHOSPHO-/3-GLUCOSIDASE GLUCOSE-1-PHOSPHATE URIDYLYLTRANS-FERASE... 

Kelley, P.M. Freeling, M. (1984a). Anaerobic expression of maize glucose phosphate isomerase I. Journal of Biological Chemistry, 259, CTh-1. 

Glucose phosphate isomerase (GPI) catalyzes the reversible interconversion of glucose-6-phosphate and fructose-6-phosphate. GPI plays an essential role in carbohydrate metabolism in all cells of the body. The substrates of this enzyme, ffuc-... 

B9. Baronciani, L., Zanella, A., Bianchi, P Zappa, M Alfinito, E, Iolascon, A., Tannoia, N., Beutler, E., and Sirchia, G., Study of the molecular defects in glucose phosphate isomerase-deficient patients affected by chronic hemolytic anemia. Blood 88,2306-2310 (1996). 

FI 3. Fujii, H., Kanno, H Hirono, A., and Miwa, S., Hematologically important mutations Molecular abnormalities of glucose phosphate isomerase deficiency. Blood Cells Mol. Dis. 22,96-97 (1996). 

XI. Xu, W. M., and Beutler, E The characterization of gene mutations for human glucose phosphate isomerase (GPI) deficiency associated with chronic hemolytic anemia. J. Clin. Invest. 94, 2326-2329 (1994). 

X2. Xu, W., Lee, P, and Beutler, E Human glucose phosphate isomerase Exon mapping and gene structure. Genomics 29,732-739 (1995). 

The reaction is irreversibly shifted to the right. Fructose 6-phosphate is isomerized to glucose 6-phosphate by glucose-phosphate isomerase. 

Glucose phosphate isomerase 5.3.1.9 C Fructose-6- phosphate NADP+ Glucose-6- phosphate Glucose-6-phosphate dehydrogenase... 

Glucose-phosphate isomerase (phosphoglucose isomerase, PGI) 700 kU/1 PGI suspension in ammonium sulfate solution. Dilute with 3.2 M ammonium sulfate to 700 kU/1 if required. 

The preparation of these solutions is described in the text in section 4.6.7.2. Pipette these successively into 1-cm cuvettes. The absorbance of the mixtures is read twice (at 340 nm) before (ODi) and after (OD2) the addition of glucose-phosphate isomerase. G6P-DH glucose-6-phosphate dehydrogenase, HK hexokinase, NADP nicotinamide adenine dinucleotide, PGI glucose phosphate isomerase, TEA triethanolamine... 

DC Kaslow, S Hill. Cloning metabolic pathway genes by complementation in Escherichia coli. Isolation and expression of Plasmodium falciparum glucose phosphate isomerase. J Biol Chem 265 12337-12341, 1990. 

Fig. 6.9. Electrophoretic patterns obtained with soluble extracts of protoscoleces removed from hydatid cysts of human(H), camel(C), sheep(S), cattle(B) and goat(G) origin from Kenya. Upper panel stained for the enzyme glucose phosphate isomerase lower panel stained for the enzyme phosphoglucomutase. (Reprinted with permission from International Journal for Parasitology, 12, Macpherson, C. N. L. McManus, D. P., A comparative study of Echinococcus granulosus from human and animal hosts in Kenya using isoelectric focusing and isoenzyme analysis, 1982, Pergamon Journals Ltd.)...

Table 6.3. Species of origin, geographical location, condition and glucose phosphate isomerase zymogram patterns of E. granulosus cyst extracts. (Data from Harrison et al., 1986)...

Faces of molecules can also be diastereotopic an example is provided by the enediol intermediate, 44, postulated to occur in the glucose-phosphate isomerase reaction ... 

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