Isolation strategies assay

To give the reader an idea of the practical effort of the immobilization strategies discussed, applications of these DNA chips are also included, e.g. with one chapter describing the immobilization step included in a short oligonucleotide ligation assay on DNA chip (SOLAC) to identify mutations in a gene of Mycobacterium tuberculosis in chnic isolates indicating rifampin resistance. 

The most successful application of structure-based predictive modeling in the future may be to specific endpoints. As described in Chapter 8 the prediction of something as broad as carcinogenicity is very difficult. If one isolates individual effects and endpoints within this category, then more success will ensue. Such an approach is likely to require the development of tiered strategies for toxicity prediction. It will also allow for the integration of test data from other assays, and where possible, human knowledge. 

Fig. (1). Peptidomics strategies used to study Drosophila immunity. (A) Using antimicrobial assays (antibacterial and antifungal), the bioactive peptides were isolated from the blood of bacteria-challenged Drosophila. MS was used for molecular mass assignment, to identify post-translational modifications, and for primary structure elucidation (B) Identification of peptidic immune effectors through differential display analysis (DD) by MALDI-MS and micro/nano RP-HPLC coupled (online) or not (off-line) to ESI-MS. When the HPLC was performed off-line to the mass spectrometer, fractions were individually analyzed by MALDI-MS. The identification and the structural characterization were performed either by molecular mass assignment and/or sequencing by ESI-MS/MS.

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