Isolation from Cryptococcus albidus

A number of heteropolysaccharides resembling that of Cr. laurentii in sugar composition have been isolated from Cryptococcus diffluens (Torulopsis rotundata) (now Cryptococcus albidus var. diffluens). ... 

Obt. by the action of endoxylanases from Cryptococcus albidus and Streptomyces on Rhodymenan a water sol. xylan isol. from the marine alga Rhadymenia palmata also by action of P-xylosidase from Penicillium wortmarmi IFO 7237 on P(1 4) xylo-biose. Cryst. 

Kooiman separated exocellular amylose formed in liquid media at pH < 5 by Cryptococcus albidus and Cryptococcus laurentii var. flavescens NRRL Y-1401 from a polysaccharide containing D-man-nose, D-xylose, and D-glucuronic acid. X-Ray diffraction patterns of the retrograded amyloses were identical with that of tuber starch (B modification). Periodate oxidation, optical rotational measurements, and hypoiodite oxidation data suggested a linear a-D-(l—>4)-linked structure having a chain length of about 44 units for the Cr. albidus amylose. Cryptococcus neoformans produces a crystalline amylose that was isolated by the method of Schoch. It has an iodine value and alpha- and hefa-amylase hydrolysis characteristics similar to those of corn amylose. 

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