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Isocitrate dehydrogenase, purification

Nimmo, G.A. Borthwick, A.C. Holms, W.H. Nimmo, H.G. Partial purification and properties of isocitrate dehydrogenase kinase/phosphatase from Escherichia coli ML308. Eur. J. Biochem., 141, 401-408 (1984)... [Pg.32]

The binding of dyes to chromatographic column material is finding widespread application in the piorification of proteins and enzymes. Thus, Nimmo and Holms describe the use of Gibacron Blue (XV) bound to Sepharose in the purification of isocitrate dehydrogenase from E.coli. °... [Pg.191]

The first demonstration of transhydrogenase activity in Pseudomonas fluoreacens by Colowick et al. (1) was carried out with a crude extract obtained from cells grown on citrate as the sole carbon source. This extract could be fractionated further by acetone precipitation followed by calcium phosphate adsorption and subsequent elution with potassium phosphate. A second acetone fractionation and calcium phosphate adsorption gave a total purification of about 200-fold. This preparation was devoid of dehydrogenase activity using glutamate, isocitrate, lactate,... [Pg.54]

See other pages where Isocitrate dehydrogenase, purification is mentioned: [Pg.6]    [Pg.103]    [Pg.290]    [Pg.455]    [Pg.104]    [Pg.155]    [Pg.54]   
See also in sourсe #XX -- [ Pg.6 ]



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