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Irreversible adsorption, practical applications

When the distribution ratio is not highly favorable, it is still possible to obtain a quantitative and selective separation through the use of a countercurrent liquid extraction approach. Although such approaches are no longer practical, having largely been supplanted by instrumental techniques such as preparative HPLC and continuous solvent extraction, countercurrent separations are conceptually useful. These approaches can be applied to preliminary separation of complex mixtures or in the isolation of compounds that do not perform well in LC because of undesirable interaction with the stationary phase (irreversible adsorption, denaturation, etc.). For these reasons, most applications of countercurrent separations involve the isolation of natural or biochemical products from plant or animal extracts. As will be described below, countercurrent extractions form the theoretical basis for LLE cartridges. [Pg.177]

For practical applications, essentially irreversible adsorption is required to prevent leaching, or desorption, which leads to activity loss. Optimum conditions are determined empirically, by varying pH, temperature, ionic strength, and quantities of protein and adsorbent. Desorption of enzymes may also be substrate induced, where high substrate concentrations cause leaching due to conformational changes. [Pg.69]

Finally, based on literature review, most retention experiments were designed for adsorption measurements where desorption data were not always sought. Therefore, kinetic retention models, such as those proposed in this study, which are capable of predicting desorption behavior of heavy in soils based solely on adsorption parameters are of practical importance. Based on our results, the overall goodness of our model predictions are considered adequate and provides added credence to the applicability of our proposed model approaches. Moreover, adsorption as well as desorption results, the second-order model was superior compared to the multireaction model. Furthermore, model formulations with consecutive irreversible retention, for MRM as well as SOTS, provided better Cu description than other model versions. [Pg.209]

TLC can be scaled-up and used for the isolation of large (10-100 mg) quantities of pure component. The practice of the technique is similar to that for analytical, qualitative scale work. The main difference lies in the plates used. Almost all preparative scale work is carried out, in the adsorption mode, principally on silica gel plates of varying thickness, 1-5 mm, and of 20 x 20 cm dimensions. The sample is applied as a streak, either by a pasteur pipette, syringe or a motorised streak applicator . Advantage can be taken of multiple development techniques, which allow efficient separation of components of markedly different polarities. Bands incompletely resolved can be applied to a fresh plate and rechromatographed with a suitable solvent and development procedure. Once development is complete the bands of component can be scraped off with a razor blade or spatula and the component washed off the adsorbent with a suitable solvent. Plates for preparative chromatography are available with added fluorescent indicator which facilitates non-destructive location of the components. The fluorescent indicator is irreversibly bound to the silica. [Pg.80]


See other pages where Irreversible adsorption, practical applications is mentioned: [Pg.136]    [Pg.69]    [Pg.32]    [Pg.257]    [Pg.153]    [Pg.147]    [Pg.655]    [Pg.53]    [Pg.67]    [Pg.22]    [Pg.122]    [Pg.19]    [Pg.403]    [Pg.230]    [Pg.48]    [Pg.235]    [Pg.164]    [Pg.618]    [Pg.311]    [Pg.50]   
See also in sourсe #XX -- [ Pg.363 , Pg.364 , Pg.365 ]




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Application adsorption

Irreversible adsorption

Practical applications

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