Ionic detergents deoxycholate

Chromatofocusing is suited for determining the isoelectric point of membrane proteins. It also provides substantial enrichment. For chromatofocusing, the protein must also be stable with low ion strength (salt interferes with focusing), and you may not use any ionic detergents such as deoxycholate, cholate, or SDS. 

Ionic detergents such as sodium dodecyl sulfate (SDS) and naturally occurring bile salts such as deoxycholate. 

Ionic detergents such as sodium deoxycholate, sarkosyl, and SDS are inhibitory to the polymerase reaction at concentrations higher than 0.06, 0.02, and 0.01% (w/v), respectively (7). 

Detergents. Under appropriate conditions of pH, ionic strength and temperature, detergents (ionic sodium lauiyl sulphate, sodium deoxycholate, sodium cholate and cetyldiethyl-ammonium bromide, or nonionic Tweens and Tritons), can be used to lyse cells. Detergents may however cause enzyme inactivation and may need to be removed before purification. 

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