Intensive reducing fish

Gdthberg A. 1983. Intensive fishing — a way to reduce the mercury level in fish. Ambio 12 259-261. 

The lipid resources of a fish are more stable than are the carbohydrates, which in muscle can be reduced by half within 15 s of intense activity (Love, 1980). Since over the longer term the carbohydrate levels are maintained by gluconeogenesis from lipids and proteins, it might seem that no meaningful information can be obtained from carbohydrate analyses, especially since, in the very act of sampling, the muscle glycogen level will fall to minute... 

FISH stains with under-digestion of tissue can be recognized due to autofluorescence in the cytosol and extracellular matrix and potential lack or reduced presence and intensity of red signals from the gene of interest (single locus). 

After mounting, the slides should be left in the dark for at least 15 minutes for DAPI to stain the nuclear material. Slides are inspected in a fluorescence microscope. It is important to use the recommended filter sets since use of sub-optimal filters significantly reduces signal intensity (see Chapter 12, Filters for FISH Imaging). 

Depending on the intensity and resolution requirement of the FISH spot, one can try binning twice. (Hint The sample will look brighter at the expense of a reduced special resolution). For more details on this topic, please visit the Microscopy Resource Center ... 

A certain amount of aluminium is mobilized through the effect of acid precipitation and ends up in streams which carry it to lakes, where all life, both plant and animal, is harmed, to the extent, in many cases, of all the fish dying out. The increase of aluminium in the water causes intense production of mucus on the surface of fish gills, the result of which is to reduce gas exchanges through the gills, leading to death by asphyxia. 

Soon after the 1917 October Revolution, some private capitalist enterprises that still existed in the Aral Sea area were affected by the destructive civil war, which meant fishing in the delta was considerably reduced. An intensive rise of the fishing industry started approximately in the 1930s. At that time all fishing industry enterprises in the Aral Sea area were integrated in the system of state fishing and fishery collective farms. 

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