Intensity and fluorescence

Strickler S J and Berg R A 1962 Relationship between absorption intensity and fluorescence lifetime of molecules J. Chem. Phys. 37 814-22... 

Since fluorescence intensity and fluorescence lifetime are both proportional to the fluorescence quantum yield, plots of If/% and t0/qt against [Q] are also linear with slope KQ and intercept 1. 

These systems possess the simplest possible variation in that only one parameter, i.e., the fluorescence quantum yield is proton-controlled. The insulation of the fluor and receptor modules in the ground state by the alkyl spacer leads to essentially pH-invariant absorption (position, shape, and intensity) and fluorescence spectra (shape and position only). The same pKa values are obtained from fluorimetry or absorption spectroscopy so that the detection sensitivity of excited state experiments can be used for the measurement of binding constants of the ground state. 

Figure 6. Changes of fluorescence intensity and fluorescence excitation spectra of compounds 2 in EPIP (a) and 6 in THF-DE (b) with irradiation time at 77 K. axexcit = in. AXmonit = ...

Axe and Weller (52) studied fluorescence and energy transfer of europium in yttrium oxide. In an experiment somewhat similar to that of Peterson and Bridenbaugh (54) on terbium, Axe and Weller were able to obtain experimental evidence for nonradiative-energy transfer between europium and other trivalent rare earth ions. Their study included both intensity and fluorescent-lifetime measurements. 

Dynamic processes at thermodynamic equilibrium that occur within a time range from sub-microseconds to seconds can be probed without the imposition of a transient disturbance by optical intensity fluctuation spectroscopy. As such, dynamic light scattering (DLS) [155] measures the fluctuation of quasielastic scattering intensity and fluorescence correlation spectroscopy (FCS) [156-158] measures concentration fluctuations of specific fluorescent molecules... 

Studies on effects of external electric fields on halobacteria with fluorescence intensity and fluorescence lifetime imaging microscopy. Chem. Lett., 37, 522-523. 

Strickler, S. J., Berg, R. A., Relationship between Absorption Intensity and Fluorescence Lifetime of Molecules, J. Chem. Phys. 1962, 37, 814 822. 

Table 2.5. Fluorescence intensity and fluorescence lifetimes of fluorescein as a function of KI...

The last two equations enable calculation of P and rj from the donor emission intensity and fluorescence lifetime data. 

Knutson et al. used NSOM to study the reactivity of nanoporous aluminium alloy 2024 [30]. In particular they used a modified version for determining concurrent topography, fluorescence intensity and fluorescence spectroscopy on alloy surface. For microelectronic and micromagnetic applications, planar and homogeneous surfaces can be formed to provide lower reactivity and longer component working fives. Typically local reactivity varies due to heterogeneous polycrystaUine and local impurities in surfaces. 

Two types of fluorescence quenching may occur, dynamic (19) and static (20). D3mamic quenching is characterized by an energy transfer to non-radiative quencher molecules. In this process, all the fluorescers are statistically affected and fluorescence intensity and fluorescer lifetime in the excited state are quantities proportional with each other. On the other hand, static quenching results from specific interactions between fluorescer and quencher molecules. Any fluorescence emission is cancelled in the case of interactions, but... 

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