Correlation between fluorescence intensity and concentration

Time-resolved spectral measurement of fluorescence during the progress of a reaction turns out to be a useful tool in photokinetics, since usually only a few of the reactants fluoresce. Fluorescence measurements have been discussed in Section 4.3. In dilute solutions a linear relationship between the fluorescence signal for a wavelength of observation a and the concentration Oi of a compound i can be found. Furthermore, fluorescence depends on the amount of light absorbed by the sample and the fluorescence 

This equation takes into account, that 

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