Integrin-ligand binding

Integrin Ligand binding assay purified receptor Functional (cell adhesion) assay... 

The structure of the integrin family has been deduced largely from the cDNA sequences of the cloned genes. All integrins comprise a- and fl-subunits, and the ligand binding that occurs at the binding site comprises an interaction between the two subunits. The a-subunits can combine with dif-... 

The possibility of binding RGD to different receptors is probably caused by a different spatial conformation of RGD due to the vicinity of different amino acids or existence of different synergistic sequences on various extracellular matrix (ECM) molecules. At the same time, one type of integrin can bind more ligands [38-40]. 

As a consequence of ligand binding and clustering of integrins, activation of different signaling pathways is initiated ... 

Meinecke, R. and Meyer, B., Determination of the binding specificity of an integral membrane protein by saturation transfer difference NMR RGD peptide ligands binding to integrin alphallbbeta3. J Med Chem, 2001, 44, 3059-3065. 

---